CHARACTERIZATION OF ENGINEERED MOUSE MODELS OF OBESITY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。神经解剖学、神经药理学和遗传学研究已经确定了中枢神经系统神经元群在体重稳态调节中起关键作用。为了评估这些中枢神经系统通路的功能意义，有必要以神经元特异性的方式“扰乱”神经肽和/或它们的受体，然后评估对能量稳态的影响。利用cre或flp介导的DNA重组(即cre/LOX和flp/flt技术)的小鼠遗传学方法为此类研究提供了手段。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Neuroanatomical, neuropharmacological, and genetic studies have identified CNS neuronal groups that are critical in the regulation of body weight homeostasis. To assess the functional significance of these CNS pathways, it is necessary to "perturb" neuropeptides and/or their receptors, in a neuron-specific fashion, and then assess effects on energy homeostasis. Genetic approaches in mice, utilizing cre- or flp-mediated DNA recombination (i.e., cre/LOX and flp/FRT technology), provide the means for such studies.