Blood and Tissue Specimen Core Facility

核心D的目标急性卒中转化研究纽约哥伦比亚合作专业项目（血液和组织核心）项目（NYCC-SPOTRIAS）提供标本处理的集中化、专业化管理，由实验室技术人员在临床病理学家实验室主任的监督下在中心机构进行实验室检测，并在受控条件下为DMA和可用于重复和额外检测的样本等分试样提供长期储存场所。这些职能将由哥伦比亚大学高级实验室医学中心（CALM）执行。据估计，在SPOTRIAS项目的整个过程中，将有680次标本采集，导致近7，000份等分试样待处理、检测 和/或存储。采集后立即将标本送至CALM进行处理、等分和DMA提取。处理后，将标本信息输入Freezerworks标本管理软件，并生成条形码标签并贴在标本上。当标本置于-80 ℃的冷冻储存条件下时，将对试管进行条形码扫描，并创建标本图以用于库存和检索。为了确保这些标本的安全性，CALM将在应急电源电路上使用一个有二氧化碳备份的监控冷冻箱。同样，DNA也会 以类似方式提取、等分并准备储存。CALM将对回收的等分试样进行hs-CRP、IL 6和TNF检测。所有检测将采用商业质控品和重复样本，按照适当的质量控制程序进行，一式两份;实验室还将订阅可用的熟练度计划。CALM工作人员将监督将样本运送到医院实验室进行CK，肝脏分析和其他必要的测试; CALM工作人员还将检索这些测试的结果，并将其输入计算机化数据库，以便转移到数据管理人员。

The aims of the Core D (Blood and Tissue Core) project of the New York Columbia Collaborative Specialized Program on Translational Research In Acute Stroke (NYCC-SPOTRIAS) are to provide centralized, professional management of specimen processing, to perform laboratory testing in a central facility by a laboratory technologist under the supervision of a Clinical Pathologist laboratory director, and to provide a place for long term storage under controlled conditions for DMA and specimen aliquots that can be used for repeat and additional testing. These functions will be performed by the Center for Advanced Laboratory Medicine (CALM) at Columbia University. It is estimated that over the course of the SPOTRIAS project, there will be 680 specimen collection episodes resulting in nearly 7,000 aliquots to be processed, tested and/or stored. Immediately after collection, specimens will be sent to CALM for processing, aliquotting, and DMA extraction. After processing, specimen information will be entered into Freezerworks specimen management software and barcode labels will be generated and placed on the specimens. As the specimens are placed in frozen storage at -80 degrees C, the tubes will be barcode wanded and a specimen map will be created for inventory and retrieval purposes. To ensure the safety of these specimens, CALM will use a monitored freezer on an emergency electrical power circuit with carbon dioxide back-up. Likewise, DNA will be extracted, aliquotted, and prepared for storage in a similar manner. CALM will perform hs-CRP, IL6, and TNF testing on retrieved aliquots. All testing will be performed in duplicate and with appropriate Quality Control procedures using commercial controls and duplicate specimens; the laboratory will also subscribe to available proficiency programs. CALM staff will oversee the delivery of specimens to the hospital laboratory for CK, Liver Profile and other tests as required; CALM staff will also retrieve the results of these tests and enter them into a computerized database for transfer to the data management staff.