Directed Evolution of EneIRED Biocatalysts for Application in Organic Synthesis

EneIREDS是最近发现的催化烯酮与胺的缀合还原/还原胺化的酶家族。为了扩大它们的合成应用，有必要设计EneIRED，使得它们具有更宽的底物范围（烯酮;胺）以及更高的立体选择性和活性。本项目的目的是应用定向进化技术开发具有广泛底物范围和高活性/选择性的野生型EneIRED变体，特别是在生物活性靶分子（包括药物）合成中的应用。该项目解决了EPSRC的关键挑战，包括可持续制造和集成催化剂的开发。流程.

EneIREDS are a recently discovered family of enzymes that catalyse conjugate reduction/reductive amination of enones with amines. In order to expand their synthetic application it is necessary to engineer EneIREDs such that they possess broader substrate scope (enone; amine) and also higher stereoselectivity and activity. Furthermore it would be advantageous to enhance their stability through immobilization.The aim of this project is to apply directed evolution technologies to develop variants of wild-type EneIREDs with broad substrate scope and high activity/selectivity, particularly for application in the synthesis of biological active target molecules including pharmaceuticals.This project addresses key EPSRC challenges including sustainable manufacturing and the development of integrated catalytic processes.