Acquisition of a MALDI-TOF High Resolution Mass Spec

描述（由申请人提供）：纽约城市大学（CUNY）是美国最大的城市大学。大约有120名教师在化学和生物化学。纽约市立大学的毕业生占少数民族博士的8%。每年都在这片土地上亨特学院/纽约市立大学质谱设施目前与整个纽约市立大学的25个实验室和包括3所医学院在内的其他教育机构的10个实验室合作。纽约市立大学系统中没有高分辨率质谱仪，导致调查人员将样本送到外面，很多时候等待相当长的时间才能得到结果。此外，很难让外部实验室进行非常规实验。为了填补这一空白，该提案要求为具有各种电离源（MALDI/ESI/APCI）的最先进的HPLC飞行时间（TOF）高分辨率质谱仪系统提供资金。拟议的系统将为亨特学院和纽约（CUNY）系统（本提案中列出的10名）的NIH研究人员提供广泛的服务。该仪器将提供小生物活性分子的经验公式确认和完整蛋白质和寡核苷酸的分子量确认。还将进行蛋白质测序分析，以鉴定蛋白质和翻译后修饰。仪器将位于亨特学院的CUNY质谱设施，并将由设施主任监督，全职博士。化学家，谁成功地管理该设施在过去10年。超过保修期的维护和供应费用将由用户费支付，这是该设施在过去7年中成功使用的机制。所要求的质谱仪将为化学、生物化学和生物科学的研究人员提供关键的科学数据。这些数据将用于帮助阐明化学结构和研究疾病的分子基础。

DESCRIPTION (provided by applicant): The City University of New York (CUNY) is the nation's largest urban university. There are approximately 120 faculty in chemistry and biochemsitry. CUNY graduates -8% of the minority Ph.D.'s in these fields yearly. The Hunter College/CUNY Mass Spectrometry Facility currently collaborates with 25 laboratories throughout CUNY, and 10 laboratories at other educational institutions including 3 medical schools. There are no high resolution mass spectrometers in the CUNY system causing investigators to send samples outside, many times waiting considerable time in order to get their results. In addition, it is difficult to get outside laboratories to conduct non routine experiments. In order to fill this void, this proposal is requesting funding for a state-of-the-art HPLC Time-of-Flight (TOF) high resolution mass spectrometer system with various ionization sources (MALDI/ESI/APCI). The proposed system will serve a broad spectrum of NIH investigators at Hunter College and the City University of New York (CUNY) system (10 listed in this proposal). The instrumentation will provide empirical formula confirmations for small bioactive molecules and molecular weight confirmations of intact proteins and oligonucleotides. Analysis of protein digests in order to identify proteins and post translational modifications will also be performed. The instrumentation will be located in the CUNY Mass Spectrometry Facility at Hunter College and will be overseen by the facility director, a full-time Ph.D. chemist, who has successfully managed the facility for the last 10 years. Maintenance and supply costs beyond the warranty period will be paid for by user fees, a mechanism that has been successfully used in this facility for the last 7 years. The mass Spectrometry instrumentation requested will provide key scientific data to researchers in the chemical, biochemical and biological sciences. This data will be used to help elucidate chemical structure and study the molecular basis for disease.