Acquisition of a MALDI-TOF High Resolution Mass Spec

描述（申请人提供）：纽约城市大学（CUNY）是美国最大的城市大学。大约有120名化学和生物化学教员。每年有8%的城市大学毕业生在这些领域获得博士学位。亨特学院/纽约市立大学质谱研究所目前与纽约市立大学的25个实验室以及其他教育机构的10个实验室（包括3所医学院）合作。在纽约市立大学系统中没有高分辨率的质谱仪，导致研究人员将样品送到外面，很多次等待相当长的时间才能得到结果。此外，很难获得外部实验室进行非常规实验。为了填补这一空白，本提案要求为具有各种电离源（MALDI/ESI/APCI）的最先进的HPLC飞行时间（TOF）高分辨率质谱仪系统提供资金。提议的系统将为亨特学院和纽约市立大学（CUNY）系统（本提案中列出的10个）的NIH研究人员提供广泛的服务。该仪器将提供小生物活性分子的经验公式确认和完整蛋白质和寡核苷酸的分子量确认。分析蛋白质消化，以确定蛋白质和翻译后修饰也将进行。该仪器将被安置在纽约市立大学亨特学院的质谱设备中，并将由设备主任（一位全职博士化学家）监督，他在过去10年里成功地管理了该设备。超过保修期的维护和供应费用将由用户费用支付，这一机制在过去7年中已在该设施中成功使用。所要求的质谱仪器将为化学、生化和生物科学的研究人员提供关键的科学数据。这些数据将用于帮助阐明化学结构和研究疾病的分子基础。

DESCRIPTION (provided by applicant): The City University of New York (CUNY) is the nation's largest urban university. There are approximately 120 faculty in chemistry and biochemsitry. CUNY graduates -8% of the minority Ph.D.'s in these fields yearly. The Hunter College/CUNY Mass Spectrometry Facility currently collaborates with 25 laboratories throughout CUNY, and 10 laboratories at other educational institutions including 3 medical schools. There are no high resolution mass spectrometers in the CUNY system causing investigators to send samples outside, many times waiting considerable time in order to get their results. In addition, it is difficult to get outside laboratories to conduct non routine experiments. In order to fill this void, this proposal is requesting funding for a state-of-the-art HPLC Time-of-Flight (TOF) high resolution mass spectrometer system with various ionization sources (MALDI/ESI/APCI). The proposed system will serve a broad spectrum of NIH investigators at Hunter College and the City University of New York (CUNY) system (10 listed in this proposal). The instrumentation will provide empirical formula confirmations for small bioactive molecules and molecular weight confirmations of intact proteins and oligonucleotides. Analysis of protein digests in order to identify proteins and post translational modifications will also be performed. The instrumentation will be located in the CUNY Mass Spectrometry Facility at Hunter College and will be overseen by the facility director, a full-time Ph.D. chemist, who has successfully managed the facility for the last 10 years. Maintenance and supply costs beyond the warranty period will be paid for by user fees, a mechanism that has been successfully used in this facility for the last 7 years. The mass Spectrometry instrumentation requested will provide key scientific data to researchers in the chemical, biochemical and biological sciences. This data will be used to help elucidate chemical structure and study the molecular basis for disease.