National User Resource for Biological Accelerator Mass Spectrometry

生物加速器质谱国家用户资源

• 批准号: 10471253
• 负责人: Graham Bench
• 金额: $ 120.67万
• 依托单位: LAWRENCE LIVERMORE NATIONAL SECURITY, LLC
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10471253
• 关键词: Address; Animal Model; Area; Award; Biochemistry; Biological; Biological Models; Biological Products; Biological Sciences; Biomedical Research; Biomedical Technology; Biophysics; Budgets; C14 isotope; Cells; Cellular biology; Code; Communities; Complex; Computer software; Data Analyses; Data Reporting; Development; Developmental Biology; Disease; Drug Kinetics; Etiology; Funding; Gases; Genetic; Goals; Grant; Health; Human; Isotopes; Label; Laboratories; Lipids; Mass Spectrum Analysis; Measurement; Measures; Metabolic; Metabolic Pathway; Metabolism; Methods; Modeling; Modification; Molecular; Molecular Biology; National Institute of General Medical Sciences; Organism; Pathway interactions; Pharmacology; Physiological; Physiology; Property; Proteins; Radioisotopes; Radiolabeled; Research; Research Personnel; Research Support; Resource Development; Resources; Risk Assessment; Safety; Sampling; Science; Scientist; Services; System; Technology; Therapeutic; Toxicology; Training; United States; United States National Institutes of Health; Work; Workforce Development; Xenobiotic Metabolism; accelerator mass spectrometry; base; carcinogenesis; detection limit; drug development; improved; in vivo; ion source; mass spectrometer; metabolic abnormality assessment; metabolic rate; nutrition; operation; programs; research study; tool; toxicant

PROJECT SUMMARY The National User Resource for Biological Accelerator Mass Spectrometry (User Resource) will provide ultra- sensitive isotopic analysis (primarily radiocarbon or Carbon-14) by accelerator mass spectrometry (AMS) for NIH-funded and other researchers. It will be the only User Resource of its type in the United States. The User Resource will leverage 20 years of expertise in the development and application of AMS in broad-based biomedical research through the Biomedical Technology Research Resource for the Development of Biomedical Accelerator Mass Spectrometry (BTRRAMS): a NIGMS Biomedical Technology Center (P41GM103483 09/01/2000 – 05/31/2020) that is in its final year of operation. Since June 2014, the BTRRAMS has measured over 20,000 samples (4,000 a year) a year for its user community. The User Resource will continue to meet the needs of current users as well as provide a capability for new users who would benefit from incorporating AMS as a measurement tool into their research programs. The User Resource’s forte will be ultra-high sensitivity quantitation of radiocarbon and selected other radioisotopes for research studies where isotopes are required. Radioisotope labeling studies continue to be an important tool for addressing many complex problems in the biomedical sciences. AMS is a specialized and unique type of mass spectrometry that provides absolute quantitation of radiocarbon and relevant other isotopes with limits of detection in real samples on the order of a few attograms/mg sample. As a National Resource, the most significant work enabled by AMS is likely to be quantification of metabolic pathways in human health states and comparison to ex vivo and in vivo animal models. The technology made available will enable a deeper understanding of the etiology of human health concerns by (1) enabling the ability to quantify pharmacokinetics and other molecular endpoints directly in humans; (2) offering the ability to conduct quantitative studies using biologics such as proteins or lipids; and (3) enabling more relevant studies of metabolic pathways in health and disease through the use of much lower, more biologically-relevant, concentrations of metabolic substrates in cells and intact organisms. Such studies support NIGMS’s basic biomedical research areas that contribute to the understanding of fundamental cellular and physiological principles. The User Resource has three goals: (1) provide high throughput, ultra-sensitive 14C analysis for the NIH user community ; (2) improve the efficiency of ultra-sensitive 14C analysis through installation of new interfaces to our AMS systems, improvements in ion source efficiency and upgrades to our data analysis codes; and (3) increase the accessibility and visibility of ultra-sensitive 14C measurements for the biomedical research community by training new investigators and expanding the National user base of researchers requiring ultra-sensitive 14C analysis.

项目摘要 国家生物加速器质谱法（用户资源）的国家用户资源将提供超级 通过加速器质谱法（AMS）的敏感同位素分析（主要是放射性碳或碳14） NIH资助和其他研究人员。它将是美国唯一类型的用户资源。用户 资源将利用在基于广泛的AMS开发和应用方面的20年专业知识 生物医学研究通过生物医学技术研究资源开发 生物医学加速器质谱法（BTRRAMS）：NIGMS生物医学中心 （P41GM103483 09/01/2000 - 05/31/2020）在运营的最后一年。自2014年6月以来 BTRRAMS每年为其用户社区测量了20,000多个样本（每年4,000个）。用户 资源将继续满足当前用户的需求，并为新用户提供能力 将AMS作为测量工具纳入其研究计划将受益。用户 Resource的Forte将是放射性碳的超高敏感性定量，并选择了其他放射性同位素 研究需要同位素。放射性同位素标签研究仍然是重要的工具 用于解决生物医学科学中的许多复杂问题。 AMS是一种专业且独特的类型 质谱法可以绝对定量放射性碳和相关的其他同位素具有极限 在实际样品中以少数图形/mg样品的顺序进行检测。作为国家资源，最多 AMS启用的重大工作可能是对人类健康状态中代谢途径的量化 并与体内和体内动物模型进行比较。提供的技术将使更深入 通过（1）能够量化人类健康关注的病因 直接在人类中的药代动力学和其他分子终点； （2）提供行为能力 使用诸如蛋白质或脂质等生物制剂的定量研究； （3）对 通过使用较低，更生物学上的较低，与生物学相关的健康和疾病中的代谢途径， 细胞和完整生物中代谢底物的浓度。此类研究支持Nigms的基本 生物医学研究领域有助于理解基本的细胞和生理 原则。用户资源有三个目标：（1）为高吞吐量提供超敏感的14c分析 NIH用户社区； （2）通过安装新的，提高超敏感14C分析的效率 与AMS系统的接口，离子源效率的提高以及升级到我们的数据分析 代码； （3）增加生物医学超敏感14C测量的可及性和可见性 研究社区通过培训新的研究人员并扩大研究人员的国家用户群 需要超敏感的14C分析。