MOLECULAR STUDIES USING AN AUTOMATED DNA SEQUENCER

描述（由申请人提供）：该提案要求资金购买AB 3730XL DNA测序仪，以取代两台老化的AB 377仪器。它将被安置在南加州大学/诺里斯综合癌症中心微化学核心实验室，该实验室为同行评审的研究人员提供DNA测序服务。获得这台新仪器将确保南加州大学的研究人员随时可以使用最先进的DNA测序技术。这对于他们在正常和肿瘤细胞中研究基因调控、信号转导和DNA修复等基本机制至关重要。此外，它还将促进对疾病遗传基础的了解，疫苗的设计和治疗癌症、移植物排斥和自身免疫的治疗方法的发展。

DESCRIPTION (provided by applicant): The proposal requests funds to purchase an AB 3730XL DNA sequencer, to replace two aging AB 377 instruments. It would be housed in the USC/Norris Comprehensive Cancer Center Microchemistry Core Laboratory, which offers a DNA sequencing service to peer-reviewed investigators. Acquisition of this new instrument will ensure that researchers at the University of Southern California have ready access to state-of-the-art DNA sequencing. This is essential in their endeavors to study fundamental mechanisms such as gene regulation, signal transduction, and DNA repair in normal and neoplastic cells. In addition, it will also advance the understanding of the genetic basis of disease, the design of vaccines and the development of therapies in the treatment of cancer, graft rejection and autoimmunity.