Robotics Extraction System/HPLC for Shared Resource Lab

描述（由申请人提供）：资助申请要求资金用于购买Gilson SPE 215自动液体处理和固相萃取/高压色谱系统，以促进维克森林大学医学院（WFUSM）高血压中心测定核心实验室对生物样品的处理和分析。目前，所有样本处理和提取均由高血压核心人员在Bridget Brosnihan博士的监督下手动进行。鉴于大量的样本和不断增长的需求，不同的RIA或ELISA程序在各种组织，血液，尿液和细胞培养，都需要大量的提取程序之前，分析，一个自动化的处理和提取系统是必要的，以保持精度，效率和输出的高血压核心实验室。此外，所要求的仪器允许将提取的样品直接进样到高效液相色谱系统（HPLC）中，用于样品和测定验证。尽管未对每份样品进行常规HPLC验证，但对单个或合并样品进行HPLC验证可确保免疫学分析中的样品鉴别，尤其是与广泛提取方法结合使用时。该核心设施为15名NIH资助的研究人员提供支持，这些研究人员持有17份NIH赠款和一个P01，其中有5个单独的项目; WFUSM研究人员提交的需要该设备的样本中，90%以上由NIH资助。高血压核心实验室提供了一个低成本，多检测设施，在一个方便的校园设置的研究人员。增加最先进的采样/提取/HPLC系统将使高血压核心实验室能够满足不断增加的样本量，并缩短向研究者输出数据的交付时间。因此，高质量的仪器，经验丰富的人员和坚实的财政支持相结合，将确保所有NIH资助的研究人员将受益于时间，数量和质量与拟议的仪器的收购。

DESCRIPTION (provided by applicant): The grant application requests funds for the purchase of a Gilson SPE 215 automated liquid handling and solid phase extraction/high pressure chromatography system to facilitate the processing and analysis of biological samples by the Hypertension Center Assay Core laboratory at the Wake Forest University School of Medicine (WFUSM). Currently all sample handling and extractions are performed manually by the Hypertension Core personnel, under the supervision of Dr. Bridget Brosnihan. Given the large amount of samples and increasing demand for different RIA or ELISA procedures in various tissues, blood, urine and cell cultures that all require extensive extraction procedures prior to analysis, an automated handling and extraction system is necessary to maintain the precession, efficiency and output of the Hypertension Core Laboratory. Furthermore, the requested instrument allows for direct injection of extracted samples into a high performance liquid chromatography system (HPLC) for sample and assay validation. Although not routinely performed for each sample, HPLC verification of either individual or pooled samples ensures sample identity in immunological analysis performed particularly when combined with extensive extraction methods. This core facility provides support for 15 NIH-funded investigators holding 17 NIH grants and a P01 with 5 individual projects; over 90% of the samples submitted by investigators at WFUSM requiring this equipment are funded by NIH. The Hypertension Core Laboratory offers a low cost, multi-assay facility to investigators in a convenient on-campus setting. The addition of the state-of-the-art sampling/extraction/HPLC system will allow the Hypertension Core Laboratory to meet the increasing sample load and reduce the delivery time of data output to investigators. Therefore, the combination of high quality instrumentation, very-experienced personnel and solid financial support will ensure all NIH-funded researchers will benefit in time, quantity and quality with the acquisition of the proposed instrumentation.