Acquisition of a MALDI-TOF Spectrometer

描述(由申请人提供)：为PE生物系统Voyager DE PRO MALDI/TOF质谱仪寻求资金。MALDI/TOF质谱仪是对多肽、蛋白质和聚合物进行质量分析的首选方法，这些分子是非挥发性和/或热不稳定的。质谱仪将安装在石溪化学生物学和药物发现研究所的分析仪器设备中。它将大大提高来自石溪的七位知名研究科学家的研究效率，他们构成了主要的用户群体。NIH资助的研究探索了控制蛋白质生物传感器光谱特性的结构-功能关系、酶催化、蛋白质催化剂的形成、蛋白质折叠、癌症治疗、配体介导的信号转导和糖基化。MALDI/TOF对于这些需要肽合成、聚合物合成、蛋白质连接和糖肽图谱的项目是必不可少的。这些样品中有许多是人工合成的。在进行进一步的实验之前，需要进行质谱分析，以验证获得了正确的产物，并分析可能发生的副反应。因此，当天的供应是我们研究企业成功的关键。其他样品很难电离，需要开发方法。这种方法的发展需要了解被调查样品的性质，投入时间寻找最佳的电离条件，并注意不浪费有限数量的样品。该仪器将由化学生物学和药物发现分析仪器研究所训练有素的人员操作和维护。该设施主任将安排日常运作，并为用户提供技术专门知识。研究支持专家将为不需要对自己的样本进行“动手”分析的用户运行样本。化学生物学研究所和药物发现指导委员会将监督该仪器的管理，确保接触到主要用户和其他NIH资助的研究人员，并管理该仪器的长期运行。石溪管理部门和化学生物学与药物发现研究所为该仪器的维护和运行做出了重大贡献。

DESCRIPTION (provided by applicant): Funding is sought for a PE Biosystems Voyager DE PRO MALDI/TOF Mass Spectrometer. MALDI/TOF mass spectrometry is the method of choice for mass analysis of peptides, proteins, and polymers, molecules that are non-volatile and/or thermally unstable. The mass spectrometer will be located in the Analytical Instrumentation Facility of the Institute of Chemical Biology & Drug Discovery at Stony Brook. It will significantly enhance the research productivity of seven established research scientists from Stony Brook who comprise the major user group. The NIH-funded studies explore the structure-function relationships that control spectroscopic properties of protein biosensors, enzyme catalysis, formation of protein catalysts, protein folding, cancer therapy, ligand-mediated signal transduction, and glycosylation. MALDI/TOF is essential for these projects that require peptide synthesis, polymer synthesis, protein ligation, and glycopeptide mapping. Many of these samples are synthetically prepared. Mass spectral analyses are required to verify that the correct product has been obtained before proceeding with further experiments, and for analyzing side-reactions that may have occurred. Thus, same day availability is key to the success of our research enterprise. Other samples are difficult to ionize, and require method development. This method development requires a knowledge of the properties of the samples under investigation, an investment of time to find the best ionization conditions, and care to not waste limited amounts of samples. The instrument will be operated and maintained by trained personnel in the Institute of Chemical Biology & Drug Discovery Analytical Instrumentation Facility. The Director of the Facility will schedule daily operation and provide technical expertise for users. A Research Support Specialist will run samples for users who do not require "hands-on" analysis of their own samples. The Institute of Chemical Biology & Drug Discovery steering committee will oversee administration of the instrument, ensure access to major users and other NIH-funded investigators, and manage long term operation of the instrument. The Stony Brook administration and the Institute of Chemical Biology & Drug Discovery have made a significant contribution toward the maintenance and operation of the instrument.