Xenogen IVIS 200 Imager

描述(由申请人提供)： 利用生物发光和荧光探针对小动物进行生物光子成像已经成为研究体内正常和疾病过程的一项最先进的技术，并为研究肿瘤生物学、传染病和基因表达提供了强有力的新途径。这项申请要求为Xenogen MS 200生物光子成像系统提供资金，以支持塔夫茨大学医学院和牙科医学院以及波士顿塔夫茨-新英格兰医学中心正在进行的广泛研究。由12名资金充足的NIH调查人员组成的核心小组将是该仪器的主要用户，并将约占其使用的79%。将建立一个小型动物成像设施来放置成像器。该设施的建立得到了生理系主席的大力支持，他为该设施提供了实验室空间，并为赠款的管理提供了支持；医学院院长将为实验室空间的翻新提供资金；塔夫茨-新英格兰医学中心负责研究的副总裁提出为仪器的维护提供资金。该设施将由PI指导，仪器将由一名全职研究助理日常操作和管理，该助理还将培训新用户。由PI以及来自塔夫茨-新英格兰医学中心、实验动物医学部和生理系的代表组成的咨询委员会将监督MS 200系统的使用，并制定使用和公平共享仪器的政策。目前，PI有一台从Xenogen借来的MS成像仪，由几个主要用户共享。然而，这台仪器必须退还给Xenogen，塔夫茨大学或塔夫茨-新英格兰医学中心没有其他合适的成像仪可用。新的MS 200成像系统和成像设备的出现将极大地加强在波士顿塔夫茨大学健康科学校区进行的研究。

DESCRIPTION (provided by applicant): Biophotonic imaging of small animals using bioluminescent and fluorescent probes has become a state-of-the-art technology for studying normal and disease processes in vivo and is providing powerful new approaches for studying tumor biology, infectious disease, and gene expression. This application requests funds for a Xenogen MS 200 biophotonic imaging system to support a wide range of research being carried out at Tufts University Schools of Medicine and Dental Medicine and the Tufts-New England Medical Center in Boston. A core group of 12 well-funded NIH investigators will be the primary users of the instrument and will account for approximately 79% of its use. A small animal imaging facility will be established to house the imager. Establishment of the facility is strongly supported by the Chairman of the Department of Physiology, who has offered laboratory space for the facility and support for administration of the grant, by the Dean of the Medical School, who will provide funds for renovation of the laboratory space, and by the Vice President of Research at Tufts-New England Medical Center who has offered funds for maintenance of the instrument. The facility will be directed by the PI and the instrument will be operated and managed on a day-to-day basis by a full time research associate who will also train new users. An advisory committee consisting of the PI, and representatives from Tufts-New England Medical Center, the Division of Laboratory Animal Medicine and the Department of Physiology will oversee use of the MS 200 system and develop policies for use and equitable sharing of the instrument. At present, the PI has an MS imager on loan from Xenogen that is being shared by several of the major users. However, this instrument will have to be returned to Xenogen and no other suitable imager is available for use at Tufts University or the Tufts-New England Medical Center. The presence of the new MS 200 imaging system and imaging facility will greatly enhance the research carried out on the Tufts University Health Sciences Campus in Boston.