800 MHZ NMR CRYOGENIC PROBE UPGRADE

描述(申请人提供)：800 MHz核磁共振低温探头升级 我们建议为密歇根大学现有的瓦里安800 MHz核磁共振系统购买一个低温三重共振探头系统(带压缩机和热交换器的冷探头)，该系统致力于为生物物理、化学、生物化学和生命科学界服务的生物大分子研究。 此次升级将服务于两个主要目的： 1)它将扩大核磁共振在研究我们研究所目前正在研究的越来越大的生物大分子及其络合物的结构、动力学和相互作用方面的适用性，以及 2)它将使我们能够更有效地满足我们不断扩大的生物分子核磁共振用户群体的各种研究人员对800 MHz系统上不断增长的核磁共振时间的需求。 800 MHz仪器将用于大(高达70 kDa)和微溶(100 FM)生物大分子及其络合物的详细溶液核磁共振研究。这些项目包括阐明变构Hsp70伴侣蛋白、线粒体ATP合成酶结构域、HIV-1RNA基因组结构域、凝集素-碳水化合物复合体、胶束溶解的膜肽和蛋白质、细菌RNase-P核糖蛋白、细菌30S核糖体核糖蛋白和细胞色素复合体的结构、动力学和相互作用。 该仪器的八名用户得到了多项NIH R01拨款的广泛支持，他们要么是核磁共振专家(3人)，要么是拥有核磁共振培训博士后的生物化学家(3人)，要么是与核磁共振专家合作的生物化学家(2人)。

DESCRIPTION (provided by applicant): 800 MHZ NMR CRYOGENIC PROBE UPGRADE We propose to purchase a cryogenic triple resonance probe system (cold-probe with compressor and heat exchangers) for the existing Varian 800 MHz NMR system at the University of Michigan, which is dedicated to biomacromolecular studies serving the Biophysics, Chemistry, Biological Chemistry and Life-Sciences communities. This upgrade will serve two broad purposes: 1) it will extend the applicability of NMR in investigating the structure, dynamics and interactions of increasingly larger biological macromolecules and their complexes currently under study at our institution, and 2) it will enable us to more effectively meet the increasing demand for NMR time on the 800 MHz system by various investigators of our expanding biomolecular NMR user community. The 800 MHz instrument will be used for detailed solution NMR studies of large (up to 70 kDa) and marginally soluble (100 fM) biomacromolecules and their complexes. The projects encompass the elucidation of the structures, dynamics and interactions of allosteric Hsp70 chaperone proteins, of domains of the mitochondrial ATP-synthase, of domains of the HIV-1 RNA genome, of lectin-carbohydrate complexes, of micelle-solubilized membrane-peptides and -proteins, of bacterial RNase-P riboproteins, of bacterial 30S ribosome riboproteins and of complexes of cytochromes. The eight users of the instrument are broadly supported by multiple NIH R01 grants, and are either NMR experts (three), biochemists with NMR-trained post-docs (three), or biochemists who collaborate with the NMR experts (two).