BD LSRII Four Laser Analytical Flow Cytometer

描述（由申请人提供）：这是一份申请资金购买贝克顿·迪金森LSRII四倍激光分析流式细胞仪的申请，供参与20多个nih资助项目的15名基础科学研究人员使用。该仪器将支持多种研究活动，包括癌症免疫学、干细胞生物学、基因治疗和发育生物学。该仪器将配备488 nm氩激光器，能够检测荧光素和藻红蛋白等标准荧光色。它还将有一个紫外线激光器，允许使用赫斯特染料外排的“侧群”方法识别干细胞。参与研究骨髓、乳腺和肝干细胞的研究人员将利用这一特性。该仪器还将配备一个氦氖激光器，允许检测额外的荧光色，如异藻蓝蛋白，这将为所有研究人员提供更大的分析能力和灵活性，以及一个紫色激光器，允许跟踪青色荧光蛋白和钙通量研究。最后，它将配备一个自动样品装载机，能够从96孔板中取样，这将有助于完成几个研究人员所追求的高通量筛选，并确保机器尽可能有效地使用，为所有研究人员提供足够的运行时间。

DESCRIPTION (provided by applicant): This is an application for funds to purchase a Becton Dickinson LSRII quadruple laser analytical flow cytometer for use by 15 basic science investigators involved in over 20 NIH-funded projects. This instrument will support a diverse group of research activities, including cancer immunology, stem cell biology, gene therapy, and developmental biology. The instrument will be equipped with a 488 nm argon laser, enabling standard fluorochromes such as fluorescein and phycoerythrin to be detected. It will also have a UV laser, allowing identification of stem cells using the "side population" method of Hoechst dye efflux. Involved investigators studying bone marrow, mammary gland, and hepatic stem cells will utilize this feature. The instrument will also have a helium-neon laser, allowing the detection of additional fluorochromes such as allophycocyanin, which will give added power and flexibility to analyses for all of the investigators, and a violet laser, allowing tracking of cyan fluorescent protein and calcium flux studies. Finally, it will be equipped with an auto sample loader, capable of sampling from 96-well plates, which will assist in accomplishing high throughput screens being pursued by several investigators, as well as ensure the machine is used as efficiently as possible, allowing sufficient running time for all investigators.