Compucyte iCys Laser Scanning Cytometer

描述（由申请人提供）：本申请申请为CSHL癌症中心的流式细胞术共享资源（2P30CA45508-14）购买一台激光扫描细胞仪（LSC）。在最简单的层面上，LSC可以被描述为流式细胞仪和倒置成像显微镜之间的交叉。LSC技术使用多达三个激光器从微量滴定板、载玻片和培养皿上的样品收集荧光和亮场图像。它允许单细胞定量的大分子和详细的动力学分析不可能与传统的悬浮流式细胞术。因此，LSC将为CSHL研究人员研究基因表达、癌症、细胞生物学和发育的调控提供一种全新的方法，CSHL的研究人员对获得这种仪器非常感兴趣，这是可以理解的。冷泉港实验室以尖端研究而闻名，但其物理隔离意味着在校园内拥有LSC等仪器对我们研究的持续成功至关重要。LSC将被安置在最近翻新的流式细胞仪设施中，并整合到现有的流式细胞仪管理和维护结构中。重要的是，流式细胞术共享资源与显微镜共享资源共用一个建筑空间，这将使我们对LSC的开发受益匪浅，这是一个自然的位置，适合这种仪器，并将促进LSC的充分利用和受益于CSHL的研究人员。

DESCRIPTION (provided by applicant): This application requests funds for the purchase of a Laser Scanning Cytometer (LSC) for the Flow Cytometry Shared Resource of the CSHL Cancer Center (2P30CA45508-14). At its simplest level, the LSC can be described as a cross between a flow cytometer and an inverted imaging microscope. LSC technology employs up to three lasers to collect fluorescent and bright-field images from samples on microtitre plates, slides and Petri dishes. It permits single-cell quantification of macromolecules and detailed kinetic analyses not possible with traditional suspension flow cytometry. The LSC will thus provide an entirely new approach to CSHL researchers studying the regulation of gene expression, cancer, cell biology, and development, and there is understandably much interest among researchers at CSHL for obtaining this instrument. Cold Spring Harbor Laboratory has a reputation for cutting-edge research, but its physical isolation means that having instrumentation such as an LSC on campus is essential to the ongoing success of our research. The LSC will be housed in the recently renovated Flow Cytometry Facility, and integrated into the existing Flow Cytometry management and maintenance structure. Importantly, our exploitation of LSC will benefit greatly from the fact that the Flow Cytometry Shared Resource shares building space with the Microscopy Shared Resource-a natural location for an instrument of this kind, and one that will promote the full use and benefits of LSC to CSHL researchers.