Protein Core

蛋白质核心的主要任务是为这三家公司提供蛋白质生产和纯化服务 本计划项目赠款的组成部分。核心将负责构建表达载体， 表达和纯化重组蛋白，提交用于生产抗体的蛋白 商业供应商，并对这些蛋白质产生的抗体进行初步评估。其核心是 还将维持一系列用于生产蛋白质的细菌和真核表达载体和细胞系。 最后，核心将存储和分发针对各种不同病毒的多克隆和单克隆抗体 核心纯化的蛋白质和结构域。这些服务将为 程序。 重组蛋白的可获得性对于实现这三个项目的目标至关重要。 程序。因为整个程序都围绕着Notch信号的共同主题，分子 在三个项目中研究的组成部分经常是共享和重叠的。因此，蛋白质 由核心设施产生的可以分发给所有三个项目组件，为每个组件提供试剂 没有重复劳动的团队。同样，为检测由以下物质产生的蛋白质而产生的抗体 核心也将被所有三个项目使用。核心设施的位置，毗邻布莱克洛 在重组蛋白的表达和纯化方面有丰富现有技能的实验室 对于生化和结构研究，将为将于年进行的项目带来额外的直接好处 Aster和Pear实验室，那里在蛋白质生产方面的专业知识较少。

The primary mission of the Protein Core is to provide protein production and purification services to the three components of this program project grant. The core will be responsible for building expression vectors, expressing and purifying recombinant proteins, submitting the proteins for production of antibodies by commercial vendors, and carrying out initial evaluation of antibodies generated to these proteins. The core will also maintain a range of bacterial and eukaryotic expression vectors and cell lines for protein production. Finally, the core will store and distribute polyclonal and monoclonal antibodies raised against the various proteins and domains purified by the core. These services will provide support to all three projects within the program. The availability of recombinant proteins will be vital to achieve the goals of all three projects within the program. Because the entire program revolves around the common theme of Notch signaling, the molecules studied in the three project components are frequently shared and overlapping. Therefore, proteins generated by the core facility can be distributed to all three project components, providing reagents to each group without duplication of effort. Similarly, the antibodies generated for detection of proteins produced by the core will also be used by all three projects. The location of the core facility, adjacent to the Blacklow laboratory where there is a wealth of existing skill in the expression and purification of recombinant proteins for biochemical and structural studies, will bring additional direct benefit to the projects to be carried out in the Aster and Pear laboratories, where there is less expertise in protein production.