THERMO ELECTRON LTQ-FT MASS SPECTROMETER

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：UCSC的13名教职员工共同开发了这份申请，以购买最先进的芬兰LTQ质谱仪系统(LTQ MS)。该系统由高性能的二维线性四极杆离子陷阱质谱仪(LTQ)组成。我们要求NCRR为购买这一系统提供资金，以极大地扩展我们的质谱学MS设施并使其现代化，该设施致力于加州大学伯克利分校化学和生物化学(CBC)、分子、细胞和发育生物学(MCD)以及环境毒理学(ETOX)系的研究。共享的MS设备目前容纳了两个质谱仪，而另外两个质谱仪被安置在单独的实验室中，并可根据要求提供给生物医学研究人员。虽然这些工具得到了很好的维护，但每一种工具都有局限性，削弱了它支持本可以在这所大学进行的研究的质量和广度的能力。如果没有额外的更新设备，我们为教员提供一流研究基础设施的能力仍将受到影响。这一问题在未来只会变得更加严重，因为这四个系的生物医学研究人员预计在未来十年内将增加50%。收购LTQ MS将在缓解这些问题方面发挥很大作用。十个主要用户的研究倡议可以分为两大领域。每一项都对基础生物科学以及相关的生物技术应用和疾病治疗产生了深远的影响。广泛的学科领域包括：1)基于ESI-线性离子陷阱的蛋白质组学来研究复杂的生化过程；2)ESI-线性离子陷阱方法来理解信号转导途径。区域1的五个主要用户(Fink、Holman、Lokey、Millhauser和Smith)在项目中使用蛋白质组学方法，这取决于拟议仪器的分辨率和灵敏度。与区域2相关的其他五个主要用户(Hartzog、Kellogg、Noller、Ottemann和Tamkun)利用这项技术来表征生化蜂窝机械。三名较小的使用者将使用该仪器研究蛋白质结构和功能，并澄清涉及毒理学的细胞机制。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Thirteen UCSC faculty members have come together to develop this application for the purchase of a state-of-the-art Finnigan LTQ Mass Spectrometer System (LTQ MS). This system consists of a high- performance 2D linear quadrupole ion trap mass spectrometer (LTQ). We are asking the NCRR to fund the purchase of this system to greatly expand and modernize our Mass Spectrometry MS Facility, which is dedicated to research in UCSC's Departments of Chemistry and Biochemistry (CBC), Molecular, Cell, and Developmental Biology (MCD), and Environmental Toxicology (ETOX). The shared MS Facility currently houses two mass spectrometers, while 2 additional mass spectrometers are housed in individual labs and available on request to the biomedical research faculty. While these instruments are well maintained, each has limitations that undercut its ability to support the quality and breadth of research that could otherwise be undertaken at this university. Without additional, updated equipment, our ability to provide our faculty with a first class research infrastructure will remain compromised. This problem will only become exacerbated in the future, as the biomedical research faculty of these four departments is projected to expand by 50% over the next decade. Acquisition of the LTQ MS will play a large role in alleviating these problems. The research initiatives of the ten major users can be divided into two broad areas. Each has far reaching implications for basic biological science, as well as for related biotechnology applications and the treatment of disease. The broad subject areas include: 1) ESI-Linear Ion-Trap based proteomics to study complex biochemical processes 2) ESI-Linear Ion-Trap approaches to understanding signal transduction pathways. The five major users in Area 1 (Fink, Holman, Lokey, Millhauser, and Smith) use proteomic methods on projects that depend on the resolution and sensitivity of the proposed instrument. Five other major users associated with Area 2 (Hartzog, Kellogg, Noller, Ottemann, and Tamkun) utilize this technology for biochemical cellular machinery characterization. Three Minor Users who will employ the instrument to investigate protein structure and function, and to clarify cellular mechanisms involved toxicology.