SMALL LAB ANIMAL IMAGING: LEUKEMIA, LUNG CANCER

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：在这份共享仪器拨款中申请了一种高分辨率微计算机断层扫描系统，MicroCAT II，由CTI分子成像公司（田纳西州诺克斯维尔）制造。MicroCAT成像系统是由能源部橡树岭国家实验室开发的，目的是加快基因工程小鼠的筛选。该系统由一个微聚焦x射线源组成，其焦斑尺寸小于10¿m，焦斑尺寸更大，用于高速研究。对于x射线探测，MicroCAT II具有高分辨率1600万像素CCD相机，具有用于筛选的大视场（FOV）和用于高分辨率成像的小视场。该系统是铅屏蔽和FDA x射线兼容。所要求的MicroCAT II系统的规格将允许nih资助的研究人员对小型实验动物和组织标本进行成像，以满足研究人员的成像需求。与Amira软件捆绑在一起的MicroCAT II系统将允许非侵入性捕获数据、生成形态信息、使用基于地标的图像配准应用程序进行纵向研究，以及疾病建模所需的高分辨率3D重建。俄亥俄州立大学没有Micro-CT成像系统。我们有其他方法来对实验动物和组织标本进行成像，然而，这些方法对结构分析不太敏感，耗时，在常规基础上使用昂贵，有不同的应用，并且不允许纵向研究。微型ct系统将最大限度地产生高质量的信息，并将使现有资源的利用更加有效和富有成效。该系统还将补充现有的小动物成像技术。MicroCAT II系统将允许美国国立卫生研究院资助的研究人员参与该计划，完成并改进他们在骨生物学、细胞周期失调和发育、成骨细胞和破骨细胞功能的转录调节、前列腺/乳腺癌和骨转移、雄激素和骨量、生长因子和骨发育、内分泌肿瘤和颅骨发育、骨组织工程、基因传递等方面的研究。MicroCAT II系统对于体内纵向研究将是重要的，避免早期牺牲有价值的研究动物。它也将对其他研究人员有用，因为它可以用来成像生物材料和软组织间期。该系统将提高检测差异的速度和能力，从而提高统计能力，使评估更多的动物成为可能，并允许体内重复测量。该仪器将放置在兽医学院的生物安全动物室（Sisson Hall），由一名研究科学家主管（专门从事成像）和一名实验动物兽医监督。已经制定了MicroCAT II成像系统的安置、维护和操作的组织和管理计划。每小时的收费将为服务合同和主管的继续教育提供资金。帐单将由兽医生物科学系的影像服务部门执行。首席研究员在橡树岭国家实验室和ImTek公司（Knoxville， TN）接受了使用MicroCAT II系统的培训。使用者将接受系统制造商、首席研究员、主管和实验动物兽医的培训。MicroCAT II控制计算机将在Windows XP环境中运行，连接到高速实时重建引擎，并连接到本地网络和俄亥俄超级计算机中心（Columbus， OH），允许从远程站点进行快速数据处理和访问。主管的工资和福利，仪器的空间和设施将由兽医生物科学系和兽医学院提供。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): A High Resolution Microcomputed Tomography System, MicroCAT II, manufactured CTI Molecular Imaging, Inc. (Knoxville, TN) is being requested in this Shared Instrumentation Grant. The MicroCAT Imaging System was developed by the Department of Energy's Oak Ridge National Laboratories with the objective of accelerating the screening of genetically- engineered mice. The system consists of a microfocus X-ray source with a <10¿m focal spot size and larger focal spots for high-speed studies. For X-ray detection, the MicroCAT II has a high resolution 16 megapixel CCD camera with a large field of view (FOV) for screening and small FOV for high resolution imaging. The system is lead-shielded and FDA X-ray compliant. Specifications in the requested MicroCAT II system will allow NIH-funded researchers to image small laboratory animals and tissue specimens, fitting the investigators imaging needs. The MicroCAT II system bundled with the Amira Software will allow the non-invasive capture of data, generation of morphological information, longitudinal studies using landmark-based image registration applications, and high resolution 3D reconstructions necessary for disease modeling. There are no Micro-CT imaging systems at the Ohio State University. We have alternate methods to image laboratory animals and tissue specimens, however, these modalities are less sensitive for structural analysis, are time consuming, expensive to be used on a routine basis, have different applications, and do not allow longitudinal studies. A microCT system will maximize the generation of high quality information and will make the use of available resources more efficient and productive. This system will also complement the existing small animal imaging technologies. The MicroCAT II system will allow the NIH-funded investigators participating in this initiative to complete and improve their studies on bone biology, cell cycle dysregulation and development, transcriptional regulation of osteoblast and osteoclast function, prostate/breast cancer and bone metastasis, androgens and bone mass, growth factors and bone development, endocrine neoplasia and skull development, bone tissue engineering, gene delivery, and others. The MicroCAT II system will be important for in vivo longitudinal studies, avoiding the early sacrifice of valuable research animals. It will also be useful to other researchers because it can be used to image biomaterials and soft tissue interphases. This system will increase the speed and ability to detect differences not possible before, thus increasing statistical power, making possible the evaluation of a larger number of animals, and allowing in vivo repeated measurements. The instrument will be located in a readily accessible biosecured animal room (Sisson Hall) of the College of Veterinary Medicine under the supervision of a Research Scientist Supervisor (specialized in imaging) and a Laboratory Animal Veterinarian. An Organizational and Administrative Plan for the placement, maintenance, and operation of the MicroCAT II Imaging System has been formulated. An hourly fee will provide funds for a service contract and continuing education for the supervisor. Billing will be performed by the Imaging Service of the Department of Veterinary Biosciences. The principal investigator has received trained on the use of the MicroCAT II system at Oak Ridge National Labs and at ImTek, Inc. (Knoxville, TN). Users will receive training by the system manufacturer, principal investigator, supervisor, and laboratory animal veterinarian. The MicroCAT II control computer will run in a Windows XP environment connected to a high-speed real-time reconstruction engine, and linked to the local networks and the Ohio Supercomputer Center (Columbus, OH), allowing rapid data processing and access from distant sites. Salary and benefits for the Supervisor and Space and utilities for the instrument will be provided by Department of Veterinary Biosciences and the College of Veterinary Medicine.