SMALL LAB ANIMAL IMAGING: GENE THERAPY, GENETICS

小型实验动物成像：基因治疗、遗传学

• 批准号: 7334991
• 负责人: RAMIRO Eliseo TORIBIO
• 金额: $ 13.73万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7334991
• 关键词: X ray; androgens; animal tissue; biomaterials; birth; bone; bone development; bone neoplasms; breast neoplasms; cell biology; cell cycle; charge coupled device camera; complement; computers; disease /disorder model; gait; gene therapy; genes; growth factor; income; lead; measurement; metastasis; molecular /cellular imaging; osteoblasts; osteoclasts; prostate; skull; tissue engineering; tissues; tomography; training; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): A High Resolution Microcomputed Tomography System, MicroCAT II, manufactured CTI Molecular Imaging, Inc. (Knoxville, TN) is being requested in this Shared Instrumentation Grant. The MicroCAT Imaging System was developed by the Department of Energy's Oak Ridge National Laboratories with the objective of accelerating the screening of genetically- engineered mice. The system consists of a microfocus X-ray source with a <10¿m focal spot size and larger focal spots for high-speed studies. For X-ray detection, the MicroCAT II has a high resolution 16 megapixel CCD camera with a large field of view (FOV) for screening and small FOV for high resolution imaging. The system is lead-shielded and FDA X-ray compliant. Specifications in the requested MicroCAT II system will allow NIH-funded researchers to image small laboratory animals and tissue specimens, fitting the investigators imaging needs. The MicroCAT II system bundled with the Amira Software will allow the non-invasive capture of data, generation of morphological information, longitudinal studies using landmark-based image registration applications, and high resolution 3D reconstructions necessary for disease modeling. There are no Micro-CT imaging systems at the Ohio State University. We have alternate methods to image laboratory animals and tissue specimens, however, these modalities are less sensitive for structural analysis, are time consuming, expensive to be used on a routine basis, have different applications, and do not allow longitudinal studies. A microCT system will maximize the generation of high quality information and will make the use of available resources more efficient and productive. This system will also complement the existing small animal imaging technologies. The MicroCAT II system will allow the NIH-funded investigators participating in this initiative to complete and improve their studies on bone biology, cell cycle dysregulation and development, transcriptional regulation of osteoblast and osteoclast function, prostate/breast cancer and bone metastasis, androgens and bone mass, growth factors and bone development, endocrine neoplasia and skull development, bone tissue engineering, gene delivery, and others. The MicroCAT II system will be important for in vivo longitudinal studies, avoiding the early sacrifice of valuable research animals. It will also be useful to other researchers because it can be used to image biomaterials and soft tissue interphases. This system will increase the speed and ability to detect differences not possible before, thus increasing statistical power, making possible the evaluation of a larger number of animals, and allowing in vivo repeated measurements. The instrument will be located in a readily accessible biosecured animal room (Sisson Hall) of the College of Veterinary Medicine under the supervision of a Research Scientist Supervisor (specialized in imaging) and a Laboratory Animal Veterinarian. An Organizational and Administrative Plan for the placement, maintenance, and operation of the MicroCAT II Imaging System has been formulated. An hourly fee will provide funds for a service contract and continuing education for the supervisor. Billing will be performed by the Imaging Service of the Department of Veterinary Biosciences. The principal investigator has received trained on the use of the MicroCAT II system at Oak Ridge National Labs and at ImTek, Inc. (Knoxville, TN). Users will receive training by the system manufacturer, principal investigator, supervisor, and laboratory animal veterinarian. The MicroCAT II control computer will run in a Windows XP environment connected to a high-speed real-time reconstruction engine, and linked to the local networks and the Ohio Supercomputer Center (Columbus, OH), allowing rapid data processing and access from distant sites. Salary and benefits for the Supervisor and Space and utilities for the instrument will be provided by Department of Veterinary Biosciences and the College of Veterinary Medicine.

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：CTI Molecular Imaging，Inc.制造的高分辨率微型计算机断层扫描系统MicroCAT II。（诺克斯维尔，田纳西州）正在请求在这个共享仪器赠款。MicroCAT成像系统是由能源部的橡树岭国家实验室开发的，目的是加速筛选基因工程小鼠。该系统由一个焦斑尺寸小于10微米的微焦点X射线源和用于高速研究的更大焦斑组成。对于X射线检测，MicroCAT II具有高分辨率1600万像素CCD相机，具有大视场（FOV）用于筛查，小视场用于高分辨率成像。该系统采用铅屏蔽，符合FDA X射线标准。要求的MicroCAT II系统的规格将允许NIH资助的研究人员对小型实验室动物和组织标本进行成像，以满足研究人员的成像需求。与Amira软件捆绑在一起的MicroCAT II系统将允许非侵入性的数据捕获、形态信息的生成、使用基于标志的图像配准应用程序的纵向研究以及疾病建模所需的高分辨率3D重建。 俄亥俄州州立大学没有微型CT成像系统。我们有其他方法来成像实验室动物和组织标本，但是，这些模式是结构分析不太敏感，是耗时的，昂贵的常规基础上使用，有不同的应用程序，不允许纵向研究。微型CT系统将最大限度地产生高质量的信息，并将使可用资源的使用更加有效和富有成效。该系统还将补充现有的小动物成像技术。MicroCAT II系统将允许NIH资助的参与该计划的研究人员完成和改进他们在骨生物学、细胞周期失调和发育、成骨细胞和破骨细胞功能的转录调节、前列腺癌/乳腺癌和骨转移、雄激素和骨量、生长因子和骨发育、内分泌肿瘤和颅骨发育、骨组织工程、基因递送等方面的研究。MicroCAT II系统对于体内纵向研究非常重要，可避免过早处死有价值的研究动物。它也将是有用的其他研究人员，因为它可以用来成像生物材料和软组织界面。该系统将提高检测差异的速度和能力，这在以前是不可能的，从而提高统计功效，使得对更多动物的评价成为可能，并允许在体内重复测量。该仪器将位于兽医学院易于进入的生物安全动物室（Sisson Hall），由研究科学家主管（专门从事成像）和实验室动物兽医监督。已经制定了MicroCAT II成像系统放置、维护和操作的组织和管理计划。每小时的费用将为主管的服务合同和继续教育提供资金。将由兽医生物科学部的成像服务部门进行计费。主要研究者在橡树岭国家实验室和ImTek，Inc.接受了MicroCAT II系统使用培训。（诺克斯维尔，田纳西州）。用户将接受系统制造商、主要研究者、监督员和实验室动物兽医的培训。MicroCAT II控制计算机将在Windows XP环境中运行，连接到高速实时重建引擎，并连接到本地网络和俄亥俄州超级计算机中心（哥伦布，OH），允许快速数据处理和远程访问。主管的工资和福利以及仪器的空间和公用设施将由兽医生物科学系和兽医学院提供。