Development of a calcium-phosphate nanocomplex fluoride-based mouthrinse

描述(申请人提供)：龋齿的形成和发展是一种疾病，到17岁时影响到近80%的美国人口。自从商业可获得的氟化物引入以来，在降低龋齿发病率方面取得了巨大的成功，但改进现有技术的机会不仅是可获得的，而且是巨大的需求。因此，找出能与氟化物协同作用的新型材料，以增强再矿化并进一步防止牙列脱矿，可能会给公众健康带来巨大的好处。含氟含漱剂是一种经过验证的良好的氟化物输送方法，特别是对高危龋齿人群，包括戴牙套、口干症和修复体的人群。因此，这项研究的长期目标是构建一种单一的多矿物摩西林配方，以显示钙磷纳米复合体和氟化物之间的稳定性，以提供比单独使用氟化物更高水平的防龋力。我们假设这些钙-磷酸盐纳米复合体可以通过机械力化学球磨技术形成，因此在氟化物中是稳定的。这种众所周知的技术不仅可以产生更小的颗粒尺寸，还可以改变研磨材料的化学特征，从而使其与通常的研磨方法截然不同。在NIDCR的第一阶段研究中，我们建议解决以下具体目标：1)使用一种易于商业应用的新型机械力化学球磨工艺，完全在固态合成多种表面活性剂-磷酸钙纳米复合体；2)测试纳米复合体添加到基本氟化钠含氟制剂中的动力学和热力学稳定性；以及3)使用pH循环再矿化/脱矿模型和釉质氟摄取研究，评估最有前景的稳定纳米复合体-氟化物含氟制剂的再矿化效果。

DESCRIPTION (provided by applicant): Caries formation and progression is a disorder that affects nearly 80% of the U.S. population by age 17. While great success has been achieved in reducing caries incidence since the introduction of commercially available fluoride, opportunities for improving the current art are not just available, they are in great demand. Therefore, identification of novel materials that could work synergistically with fluoride to enhance remineralization and further prevent demineralization of the dentition could provide a tremendous public health benefit. Fluoride mouthrinses are a proven and favorable method of fluoride delivery, especially for high-risk caries groups including those wearing braces, those with xerostomia, and those with restorations. Therefore, the long-term goal of this research is to construct a single multi-mineral mouthrinse formulation that manifests stability between calcium-phosphate nanocomplexes and fluoride to provide increased levels of protection against caries than would be observed with fluoride alone. We posit these calcium-phosphate nanocomplexes can be formed through a mechanochemical ball milling technique and, as a result, are stable with fluoride. This well-known technique not only creates smaller particle sizes but also modifies the chemical signatures of the milling material, thus setting it distinctly apart from usual milling methods. In this Phase I research application to the NIDCR, we propose to address the following Specific Aims: 1) Synthesize numerous surfactant-calcium phosphate nanocomplexes entirely in the solid-state using a novel mechanochemical ball milling process that is readily scaleable for commercial applications; 2) Test the kinetic and thermodynamic stability of the nanocomplex when added to basic sodium fluoride mouthrinse formulations; and 3) Evaluate the remineralization efficacy of the most promising stable nanocomplex-fluoride mouthrinse formulations using pH cycling remineralization/demineralization models and enamel fluoride uptake studies.