Real-time nucleic acids detection assay with enhanced 5'-nuclease activity

描述(由申请人提供)：已经发现了增强FRET-探针切割的简单和直接的方法。这一阶段研究的主要目标是建立一种新的实时荧光聚合酶链式反应方法，用于核酸的检测和鉴定。初步结果表明，该技术有效地与其他已知的FRET-探针系统竞争，在实时PCR中提供了强大的信号性能。新的分析方法有望成为基因组研究，特别是单核苷酸多态鉴定和基因分型的一种优秀工具。它可以有效地应用于研究和临床环境中的许多应用，包括疾病连锁研究、种群分析、表达谱和病原体鉴定。该项目的主要目标是开发一种新的、改进的实时荧光聚合酶链式反应方法，用于核酸检测和鉴定。我们的初步研究表明，该技术将成为人类基因组学中的一种优越工具，并可有效地应用于研究和临床环境中的许多应用，包括疾病连锁研究、群体分析、基因分型、表达谱分析和病原体鉴定。

DESCRIPTION (provided by applicant): Simple and straightforward approach to enhance cleavage of FRET-probes has been discovered. Main objective of this Phase I study is to develop a novel real-time PCR assay for nucleic acid detection and identification. Preliminary results indicate that the technology effectively competes with other known FRET-probe systems providing strong signal performance in real-time PCR. New assay is anticipated to become a superior tool for genomic studies and, in particular, for single nucleotide polymorphism identification and genotyping. It can be effectively used in research and clinical environment for numerous applications including disease linkage study, population analysis, expression profiling and pathogen identification. Main objective of the proposed project is to develop a novel and improved real-time PCR assay for nucleic acid detection and identification. Our initial study indicates that the technology will become a superior tool in human genomics, and it can be effectively used in research and clinical environment for numerous applications including disease linkage study, population analysis, genotyping, expression profiling and pathogen identification.