Fluorescence-activated cell sorter-FACSAria

描述(由申请人提供)：我们请求支持BD生物科学FACSAria“荧光激活细胞分类器(FACS)”。这台机器可以进行流式细胞仪分析，并可以根据荧光对细胞进行分类。这是一种最先进的仪器，它结合了高吞吐量性能和相对易用性和低维护。FACSAria机器将放置在一个新的化学和生命科学学院成像设施中，该设施将位于马里兰大学学院公园校园内一座计划于2007年5月开放的新建生物科学研究大楼内。这台仪器将由一名全职操作员操作，该操作员将由大学雇用并提供全面支持。荧光激活的细胞分类器将设在生命科学学院内，但所有大学研究人员都可以使用。马里兰大学对生命科学的扩展和改进做出了重大承诺。人们普遍认识到，随着这一扩张，需要新的核心设施，并雇用一名经验丰富的科学家来管理这些设施。荧光激活细胞分选是一种重要的实验方法，它允许研究人员识别复杂混合物中的细胞亚群，并根据特定的荧光强度将它们分类为均一群。FACS在整个生命科学中都被使用，FACSAria分类流式细胞仪将被整个大学的许多研究人员使用。这将是整个校园中唯一的荧光激活细胞分选器，该校拥有1500多名研究人员。因此，大学同意作出重大承诺，支持购买这一工具。CLFS院长诺玛·阿勒韦尔博士已承诺10万美元购买这台仪器，并100%支持操作员的工资。该申请是代表新成立的马里兰病原体研究所(MPRI)提交的，该研究所位于生命科学学院内，但作为校园内的一个多学科研究机构，包括来自位于大学内4个不同学院(生命科学、工程、健康与人类表现、兽医)的8个不同系(CBMG、BIO、CBR、MOCB、CHHP、CVM、Mtech、ANSC)的研究人员。这台仪器的加入将大大增强校园内跨越多个学科的各种科学项目，包括病原体研究、免疫学、人类行为和神经生物学。

DESCRIPTION (provided by applicant): We are requesting support for a BD Biosciences FACSAria" fluorescence activated cell sorter (FACS). This machine can perform flow cytometric analysis, and it can sort cells on the basis of fluorescence. This is a state of the art instrument that combines high throughput performance with relative ease of use and low maintenance. The FACSAria machine will be placed in a new College of Chemical and Life Sciences Imaging Facility which will be located in a newly constructed Biosciences Research Building scheduled to open in May 2007 on the campus of the University of Maryland, College Park. This instrument will be operated by a full time operator who will be hired and fully supported by the University. The fluorescence activated cell sorter will be located within the College of Life Sciences but it will be available to all university investigators. The University of Maryland has made a major commitment to expansion and improvement in the Life Sciences. It is widely recognized at the University that with this expansion, comes the need for new core facilities and the hiring of an experienced scientist to run these facilities. Fluorescence activated cell sorting is an important experimental approach which allows investigators to identify sub-populations of cells within a complex mixture, and sort them into a homogenous populations, based on specific fluorescence intensities. FACS is used throughout the life sciences, and the FACSAria sorting flow cytometer will be used by many investigators throughout the University. This would be the only fluorescence-activated cell sorter on the entire campus, which has a faculty in excess of 1500 investigators. Consequently, the University has agreed to make a major commitment to support the purchase of this instrument. Dr. Norma Allewell, Dean of CLFS, has committed $100k toward the purchase of this instrument, and 100% support of the operator's salary. The application is being submitted on behalf of the newly formed Maryland Pathogen Research Institute (MPRI), which is physically located within the College of Life Sciences, but as a multidisciplinary research institute on campus, includes investigators from 8 different Departments (CBMG, BIO, CBR, MOCB, CHHP, CVM, MTECH, ANSC) located in 4 different Colleges (Life Sciences, Engineering, Health & Human Performance, Veterinary Medicine) within the University. A variety of scientific programs on campus spanning a number of disciplines, including pathogen research, immunology, human performance, and neurobiology will be substantially enhanced by the addition of this instrument.