Biacore T100

描述(由申请人提供)：该合同将支持亚利桑那州立大学收购Biacore T100分子相互作用分析系统。T100仪器利用表面等离子体共振(SPR)技术，将补充目前在园区使用的两个SPR仪器，Biacore X和FlexChip，这两个仪器都没有能力或能力来满足亚利桑那州立大学日益增长的表征分子相互作用的需求。亚利桑那州立大学的增长包括在过去两年中净增加125名终身教职员工和终身教职员工，同时科学研究也相应增长。亚利桑那州立大学生物设计研究所是一家在科学学科合并方面令人兴奋的企业，拥有来自生物、化学、物理、医学、农业、环境科学、电子、材料科学、工程和计算等领域的教员。Biacore T100提供了自动化、改进的软件和灵敏度，与校园中现有的两种仪器中的任何一种相比，进行进一步分析所需的自动化、改进的软件和灵敏度。仪器将放置在生物设计研究所，所有实验室位于大楼内的PI都可以很容易地接触到它，并且离其他建筑中的PI很近。我们计划招聘一名具有SPR经验的博士后担任该设施的经理。经理的工资支持最初将由该工具的主要用户分担，但最终将从该工具产生的使用费中支付。亚利桑那州立大学在校园内拥有多个可回收的核心设施，已成功运行5-10年(如DNA实验室、蛋白质化学实验室、EM实验室)。文书的时间表将是电子的，冲突将由一个咨询委员会解决，该委员会由赠款的PI、两个主要用户和一个次要用户组成。大约15%的可用时间将留给新用户和外部用户。我们目前已将6名研究生送到比亚科雷进行培训，这些实验室自培训以来已经能够产生初步数据。我们计划在仪器在校园交付后派遣更多的人员进行培训。尽管我们可以使用Biacore X，但它的使用率已经很高(两个主要用户超过50%)，无法满足我们不断扩大的教员的需求。位于校园的FlexChip上也产生了初步数据，但进一步的分析需要BioCore T100提供的更高的灵敏度。我们的需求已经远远超出了目前可用的仪器可获得性，而购买T100将极大地提前研究时间表。公共卫生相关性：我们在这里描述的项目包括疫苗学、癌症和疾病治疗、神经疗法和生物防御；了解生物分子如何相互作用是成功寻求全球公共卫生官员每天面临的相关问题答案的先决条件。

DESCRIPTION (provided by applicant): This award will support the acquisition of a Biacore T100 molecular interaction analysis system at Arizona State University. The T100 instrument, which utilizes surface plasmon resonance (SPR) technology, will complement the two SPR instruments currently in use on the campus, a Biacore X and a FlexChip, neither of which has the capabilities or capacity to satisfy the growing needs for characterizing molecular interactions at ASU. Growth at ASU has included a net increase of 125 tenured and tenure-track faculty during the past two years with a corresponding growth in scientific research. The Biodesign Institute at Arizona State University is an exciting venture in merging of scientific disciplines, with faculty from the fields of biology, chemistry, physics, medicine, agriculture, environmental science, electronics, materials science, engineering and computing. The Biacore T100 offers the automation, improved software, and sensitivity that is required to take analysis further than is possible with either of the two instruments currently available on campus. The instrument will be housed at The Biodesign Institute, where it will be readily accessible to all PIs whose labs are located in the building, and is in close proximity to PIs located in other buildings. We plan to recruit a postdoctoral fellow with SPR experience to be the manager of the facility. The salary support for the manager will be initially shared by the major users of the instrument, but will eventually be paid from the usage fees generated by the instrument. ASU has several recoverable core facilities on campus which have operated successfully for 5-10 years (such as DNA Laboratory, Protein Chemistry Lab, EM Lab). Scheduling of the instrument will be electronic, and conflicts will be resolved by an advisory committee consisting of the PI of the grant, two major users, and one minor user. Approximately 15% of the available hours will be reserved for new and outside users. We currently have sent 6 graduate students to Biacore for training, and those labs have been able to generate preliminary data since the training. We plan to send additional personnel to training when the instrument has been delivered on campus. Though we have some access to the Biacore X, it is heavily utilized already (greater than 50% by two major users) and cannot fill the needs of our expanding faculty. Preliminary data has also been generated on the FlexChip located on campus, but further analysis requires the greater sensitivity available with a Biocore T100. Our needs have far outgrown the currently available instrument accessibility, and the purchase of the T100 will greatly advance the research schedules. Public Health Relevance: Our herein described projects encompass vaccinology, cancer and disease treatments, neurological therapies, and biodefense; an understanding of how biological molecules interact is a prerequisite to successful pursuit of answers to the relevant problems faced each day by Public Health officials globally.