Acquisition of a 500 MHz NMR Spectrometer

描述（由申请人提供）：在本提案中，要求资金购买一台500 MHz核磁共振光谱仪，以支持康奈尔大学威尔医学院的生物医学研究。该仪器将直接支持16项NIH资助的研究补助金，并作为医学院的核心资源。目前，该大学拥有一台核磁共振光谱仪，专门用于蛋白质的核磁共振结构分析。在威尔-康奈尔大学，对合成化合物、天然产物和小肽的分析的需求日益增长，用于各种生物和医学研究。所要求的仪器将大大减轻唯一光谱仪的负担，提供生物和合成化学的分析能力，并支持新建立的化学合成核心设施。核磁共振波谱仪将优化小分子和肽的研究。核磁共振将包括一个逆1H/13C/15N探针，具有三个射频通道，可进行三重共振实验。该仪器将具有威尔-康奈尔目前不存在的功能，并且在当地无法使用。这些包括：1)用于直接检测1H、19F、31P、11B、13C、23Na和15N的宽带探针；2)用于自动样品插入和数据采集的多采样器；3)数据收集的温度范围从-150°C到180°C。该仪器将位于核磁共振核心设施，这是康奈尔大学威尔医学院研究人员可用的分析核磁共振的唯一来源。

DESCRIPTION (provided by applicant): In this proposal, funds are requested to acquire a 500 MHz NMR spectrometer to support biomedical research at Weill Medical College of Cornell University. The instrument will directly support 16 NIH funded research grants and serve as a core resource for the medical college. Currently, the university has one NMR spectrometer dedicated for NMR structural analysis of proteins. There is a growing need at Weill-Cornell for analysis of synthetic compounds, natural products and small peptides for a variety of biological and medicinal studies. The requested instrument will significantly reduce the burden on the sole spectrometer, provide analytical capability for biological and synthetic chemistry, and support the newly established Chemical Synthesis Core facility. The NMR spectrometer will be optimized for both small molecule and peptide studies. The NMR will include an inverse 1H/13C/15N probe with three radio frequency channels enabling triple resonance experiments. The instrument will have capabilities that do not currently exist at Weill-Cornell and are unavailable locally. These include: 1) a broad-band probe for direct detection of 1H, 19F, 31P, 11B, 13C, 23Na, and 15N; 2) a multi-sampler for automated sample insertion and data acquisition; and 3) an expanded temperature range for data collection from -150 ¿C to 180 ¿C. The instrument will be located in the NMR Core facility, the only source of analytical NMR available to researchers at Weill Medical College of Cornell University. Relevance: NMR spectrometers are essential for molecular structure determination and chemical identification. The acquisition of a 500 MHz NMR will directly support NIH sponsored research aimed at understanding and combating bacterial infection, malaria, AIDS, cancer, tuberculosis, Alzheimer's, Parkinson's and cardiovascular diseases. The instrument purchased will be employed to identify and characterize proteins and peptides involved in disease processes that might serve as pharmaceutical targets, as well as synthetic molecules that influence bioactivity.