Vaccine delivery: Characterization of vaccine delivery and metabolic impact investigation using secondary ion mass spectroscopy

使用负载纳米颗粒来表达预防性抗原和治疗性蛋白，开启了医疗保健领域的新一代生物技术，使用非病毒递送载体来安全和有效地递送外源基因有效载荷(例如，用于抗击人畜共患传染病)。有效地提供疫苗必须克服一系列生理和生物障碍，目前我们对这些障碍的了解是有限的。该项目致力于使用最先进的表征技术，如质谱学成像，来解决这些限制。合成纳米颗粒是一种输送载体，它具有细胞内不存在的独特成分。当使用二次离子质谱仪等表征技术时，这些成分将被用来跟随运送工具，并为纳米颗粒提供化学“指纹”。这将在这个项目中用于通过绘制递送工具的化学指纹和外源疫苗机制的代谢影响来提高我们询问生理环境的能力。与葛兰素史克密切合作，该项目取得的成果将为改进疫苗和递送载体的设计提供关键的机械证据，通过洞察递送如何发生和影响细胞环境。

The use of loaded nanoparticles to express prophylactic antigens and therapeutic proteins has initiated a new generation of biotechnology in healthcare using non-viral delivery vehicles to safely and potently deliver the exogenous genetic payloads (e.g. to combat infectious diseases of a zoonotic origin). Effective delivery of vaccines must negotiate a series of physiological and biological barriers for which our understanding is presently limited. This project aspires to address these limitations using state of the art characterisation techniques such as mass spectrometry imaging. Synthetic nanoparticles are delivery vehicles that have unique components that are not endogenously present in cells. These components will be used to follow the delivery vehicle and provide a chemical "fingerprint" for the nanoparticle when using characterization techniques such as secondary ion mass spectrometry. This will be used in this project to improve our capacity to interrogate the physiological environments by mapping the chemical fingerprint of the delivery vehicle and metabolic impact of exogenous vaccine mechanisms. Working closely with GSK, the results achieved in this project will provide key mechanistic evidence to improve the design of vaccine and delivery vehicles by providing insight regarding how delivery occurs and influences the cellular environment.