Behavioral actions of angiotensin II: Circuits and intracellular signals

描述(由申请人提供)： 项目总结：候选人、环境和研究。这份提案描述了一项为期5年的培训计划，旨在为应聘者提供所需的专业知识，以实现他作为一所学术研究大学的独立行为神经学家的职业生涯的长期目标。这位候选人于2001年获得博士学位，此后一直在宾夕法尼亚大学动物生物系和马奥尼神经科学研究所担任博士后研究员。马奥尼神经科学研究所容纳了来自宾夕法尼亚大学18个系和6个学院的180多名教职员工，为候选人提供了理想的培训环境。拟议的导师史蒂文·J·福尔哈蒂博士是行为神经科学领域的知名研究人员，也是拟议实验中研究的荷尔蒙--有效的血管紧张素II(AngII)--的细胞和行为反应方面的专家。该研究计划的重点是水、盐和食物的摄入量，这些都是体液、心血管和能量稳态的关键成分。将这些行为理解为独立的、但相关的摄入行为形式，可能会揭示对它们的控制和调节的新见解。这里描述的实验旨在确定与摄食行为的荷尔蒙刺激相关的细胞内事件。血管紧张素转换酶受体产生一系列不同的细胞内反应，然而这些反应与血管紧张素转换酶治疗产生的行为之间的联系尚不清楚。这里描述的实验利用新的药理学方法来确定这些不同的细胞内反应与血管紧张素转换酶诱导的摄食行为的行为相关性，并探索一般摄食行为的共性。 项目摘要：与公共卫生的相关性。摄入水、盐和食物对维持体内平衡至关重要。影响体液、心血管和能量平衡的疾病包括高血压、心脏病或肾脏疾病、糖尿病和肥胖。确定激素调节这些行为的神经机制对于全面理解这些现象至关重要，并可能导致对抗相关疾病状态的新的治疗方法。

DESCRIPTION (provided by applicant): Project summary: candidate, environment, and research. This proposal describes a 5-year training program designed to provide the candidate with the expertise needed to achieve his long-term goal of developing a career as an independent behavioral neuroscientist at an academic research university. The candidate received a Ph.D. in 2001 and has since been conducting research as a postdoctoral fellow in the department of Animal Biology and the Mahoney Institute for Neurological Sciences at the University of Pennsylvania. The Mahoney Institute for Neurological Sciences houses over 180 faculty members from eighteen departments and six schools within the University of Pennsylvania and provides an ideal training environment for the candidate. The proposed mentor, Dr. Steven J. Fluharty, is a well-established researcher in the field of behavioral neuroscience and an expert in the cellular and behavioral responses to the hormone studied in proposed experiments, the potent dipsogen angiotensin II (AngII). The research plan focuses on water, salt, and food intake, key components of body fluid, cardiovascular, and energy homeostasis. Understanding these behaviors as separate, but related forms of ingestive behavior has the potential to reveal novel insights into their control and regulation. The experiments described here are designed to determine the intracellular events associated with the hormonal stimuli for ingestive behavior. AngII receptors produce a diverse array of intracellular responses, yet the connection between any of these responses and the behaviors produced by treatment with AngII remains unclear. The experiments described here take advantage of novel pharmacological approached to determine the behavioral relevance of these divergent intracellular responses to AngII-induced ingestive behaviors and explore commonalities of ingestive behaviors in general. Project summary: Relevance to public health. Ingestion of water, salt, and food is critical for maintenance of homeostasis. Disorders affecting body fluid, cardiovascular, and energy homeostasis include hypertension, heart or kidney disease, diabetes, and obesity. Determining the neural mechanisms through which hormones mediate these behaviors is critical for a complete understanding of the phenomena and may lead to novel therapeutic approaches to combat the relevant disease states.