Structural and functional studies of ion transporters.

描述（申请人提供）：离子共转运蛋白是介导两种不同离子跨膜共转运的膜蛋白。它们在人体生理学中发挥重要作用;例如，Na+/i-同向转运体负责甲状腺中I-的积累。尽管它们的重要性，一个详细的机械理解如何协同转运蛋白的工作是缺乏的。本研究的长期目标是建立一个详细的分子模型，研究Na+和K+通过细菌同向转运蛋白KtrAB的共转运。 这项建议的主要目的是： 1 -表征KtrAB同向转运体的运输性质。 2.描述KtrA的结构和功能。 3 -确定膜蛋白KtrB的晶体结构。

DESCRIPTION (provided by applicant): Ion symporters are membrane proteins that mediate the co-transport of two different ion species across themembrane. They play important roles in human physiology; the Na+/i- symporter, for example, is responsible for accumulation of I- in the thyroid gland. Despite their importance a detailed mechanistic understanding of how symporters work is lacking. The long term objective of this proposal is the development of a detailed molecular model for the co-transport oftwo ions, Na+ and K+ by KtrAB, a bacterial symporter. The major aims of this proposal are: 1 - Characterize the transport properties of the KtrAB symporter. 2 - Characterize the structure and function of KtrA. 3 - Determine the crystal structure of the membrane protein KtrB.