Targeted, multimodal detection of carbon monoxide and other markers of disease in cells using fluorogenic probes

尽管一氧化碳（CO）有毒，但它也是一种重要的生物信使分子，调节许多重要的细胞过程，包括对疾病的反应。一氧化碳的酶促生成增加在炎症过程的解决和心血管疾病的缓解中起着关键作用。与此同时，粘度水平的改变与炎症有关，包括心血管疾病。该项目将开发一系列钌（II）和锇（II）一氧化碳分子探针，并已用于监测动物模型中的炎症。由于一氧化碳的扩散控制运动也受到细胞环境粘度变化的影响，这两个方面是密切相关的。因此，同时测量一氧化碳和其他疾病标志物，如细胞内粘度，提供了有关细胞功能和炎症及疾病状态的前所未有的信息。

Despite its toxic reputation, carbon monoxide (CO) is also an important biological messenger molecule that regulates many vital cell processes, including the response to disease. Increased enzymatic generation of carbon monoxide plays a critical role in the resolution of inflammatory processes and alleviation of cardiovascular disorders. At the same time, altered viscosity levels have been associated with inflammation, including in cardiovascular disease. This project will develop a range of ruthenium(II) and osmium(II) molecular probes for carbon monoxide and has used them to monitor inflammation in an animal model. Since the diffusion-controlled movement of carbon monoxide is also affected by changes in the viscosity of the cellular environment, the two aspects are intimately connected. Therefore, the simultaneous measurement of both carbon monoxide and other markers of disease, such as intracellular viscosity, provides unprecedented information on the functioning of the cell and the state of inflammation and disease.