Characterization of the cichlid orange blotch mutant

描述(由申请人提供)：使用多能干细胞的再生疗法有望治疗各种病理疾病。然而，决定发育期间祖细胞组织的生长、构型和动态平衡的机制与那些参与成年生物体组织再生的机制相比，仍然很大程度上未知。鱼类中的色素模式代表了一个有吸引力的模型系统，可以直接显示成鱼的模式机制，因为鱼类经历了幼体到成体的变态，这涉及到色素细胞种群的生长和组织的变化，而再生鳍的能力允许在组织再生期间检查色素细胞生物学。这项拟议的研究旨在鉴定自然发生的慈鱼橙斑突变体。在成体发育和再生过程中，色素细胞的迁移和分化将通过空间和时间分析进行对比分析，并利用谱系标记对分化状态进行原位表征。此外，橙斑病基因将在先前定位的区间内使用定向定位方法和候选基因分析进行鉴定。

DESCRIPTION (provided by applicant): Regenerative therapy utilizing pluripotent stem cells holds promise for treatment of a variety of pathological conditions. However, the mechanisms that determine the growth, patterning, and homeostasis of tissue from progenitor cells during development versus those involved in tissue regeneration in an adult organism remain largely unknown. Pigment pattern in fishes represents an attractive model system to directly visualize mechanisms of patterning in adults, since fish undergo larval to adult metamorphosis that involves changes in growth and organization of pigment cell populations, and the capacity to regenerate fins permits examination of pigment cell biology during tissue regeneration. The proposed research aims to characterize the naturally occurring cichlid fish orange blotch mutant. Pigment cell migration and differentiation will be comparatively analyzed during adult development and regeneration using spatial and temporal analysis, as well as in situ-based characterization of differentiation state with lineage markers. Also, the orange blotch gene will be identified within the previously mapped interval using a directed mapping approach and candidate gene analysis.