A Genetic Mapping Resource for Zebrafish

描述（由申请人提供）：斑马鱼突变筛选有望识别与发育和疾病相关的表型基因。关联表型和基因型将需要开发与这些筛选的规模在通量上匹配的基因分型工具。这项建议解决了这一差距，以回应该计划宣布“工具遗传研究斑马鱼。“我们建议应用我们在高通量基因分型方面的丰富经验，为斑马鱼社区创建共享的全基因组图谱资源。主要研究者开发了最先进的基因分型技术和信息学工具，目前可直接应用于斑马鱼作图。我们已经完成了约100万个基因型，开发了超过75个荧光简单串联重复序列（STR）标记组（包括用于人类遗传图谱的强大全基因组），在大规模人类研究中发现并基因分型了候选基因的综合单核苷酸多态性（SNP）图谱，现在正在进行全基因组小鼠SNP扫描。在这里，我们提出了共享斑马鱼映射资源的发展：强大的全基因组标记集的高通量遗传筛选，和一个公共的生物信息学系统，组织和设计定制的检测随后的精细映射。

DESCRIPTION (provided by applicant): Zebrafish mutagenesis screens hold promise for identifying genes underlying phenotypes relevant to development and to disease. Associating phenotype and genotype will require development of genotyping tools matched in throughput to the scale of these screens. This proposal addresses this gap in response to the program announcement "Tools for Genetic Studies in Zebrafish." We propose to apply our extensive experience in high-throughput genotyping to create a shared genome-wide mapping resource for the zebrafish community. The Principal Investigator has developed state-of-the-art genotyping technologies and informatics tools that are currently operational and directly applicable in zebrafish mapping. We have completed approximately one million genotypes, developed >75 fluorescent simple tandem repeat (STR) marker panels (including a robust genome-wide set for human genetic mapping), discovered and genotyped comprehensive single nucleotide polymorphisms (SNP) maps across candidate genes in large-scale human studies, and are now performing a genome-wide mouse SNP scan. Here we propose development of shared zebrafish mapping resources: robust genome-wide marker sets for high-throughput genetic screens, and a public bioinformatics system to organize and design custom assays for subsequent fine mapping.