High Throughput Tools for Mammilian CellCulture Bioprocessing and Analysis

该项目旨在设计和建立一个灵活的机器人平台，以自动孵化和处理在摇瓶中生长的数百种哺乳动物细胞培养物，并集成细胞生长和代谢物分析。摇瓶模拟大型生物反应器，有足够的体积进行频繁取样分析和支持工艺开发和细胞系稳定性研究。目标是确保高通量生物过程研究的一致，可重复的细胞处理。这将有助于系统地、平行地研究影响细胞生长或生产力的多个参数，例如培养基配方或饲料制度。“智能”软件将被开发出来模拟培养过程并模仿培养专家，从而自动决定如何以及何时处理每个个体培养，例如继代培养到目标细胞计数，或者何时喂食以维持设定的葡萄糖水平。

The project seeks to design and build a flexible robotic platform to automate incubation and processing of hundreds of mammalian cell cultures growing in shake flasks, with integrated cell growth and metabolite analysis. Shake flasks model larger scale bioreactors, have ample volume for frequent sampling for analysis and support process development and cell line stability studies.The goal is to ensure consistent, reproducible cell processing for high throughput bioprocess research. This would facilitate systematic, parallel investigation of multiple parameters influencing cell growth or productivity, e.g. media formulation or feed regime."Smart" software would be developed to model the process and mimic the culture expert, and thus automatically decide how and when to process each individual culture e.g. subculture to a target cell count, or when to feed to maintain a set glucose level.