MOLECULAR BIOLOGY

分子生物学核心的主要作用是：(1)为分子生物学的利用提供服务 (2)促进将分子生物学的进展落实到IBD研究中。应用 分子生物学方法是评估IBD发展过程的核心 根据作为该中心基础的假设。这些方法包括评估 维持上皮功能和完整性的机制、调节免疫激活和过程的因素 有助于放大炎症反应和组织损伤[1]。最重要的是，分子 生物学方法在研究先天性免疫中是必不可少的，宿主：粘膜中的微生物相互作用 界面、树突状细胞/免疫细胞相互作用、人类遗传评估和IBD小鼠模型 它们构成下一个支持期内IBD相关研究的关键组成部分[2-8]。很明显，这是从 大多数中心研究人员使用基本分子生物学技术作为CSIBD研究基地的描述 他们的研究项目的基本组成部分。这些技术允许识别和表征 调节上皮细胞和免疫细胞功能的基因，这些基因的表达分析， 功能有助于Tie编码蛋白的相互作用，报告基因和蛋白质的表达，以允许细胞定位和生理分析。

The major roles of the Molecular Biology Core are (1) to provide services in the utilization of molecular biological techniques and (2) to facilitate the implementation of advances in molecular biology to IBD research. Application of molecular biological approaches is central to the evaluation of processes underlying the development of IBD according to the hypotheses that serve as a foundation for this Center. These approaches include evaluation of the mechanisms for maintaining epithelial function and integrity, factors regulating immune activation and processes contributing to amplification of inflammatory responses and tissue injury [1]. Most importantly, molecular biological approaches are essential in the study of innate immunity, host:microbial interactions at the mucosal interface, dendritic cell/immune cell interactions, human genetic evaluation and murine models of IBD, areas which form key components of IBD-related research in the next support period[2-8]. It is apparent from the description of the CSIBD research base that most Center investigators use basic molecular biological techniques as a fundamental component of their research projects. These techniques permit the identification and characterization of genes regulating epithelial and immune cell function, analysis of the expression of these genes, determination of the functions aid interactions of tie encoded proteins, expression of reporter genes and proteins to allow cellular localization and physiological analysis.