CYTOHISTOCHEMISTRY CORE

描述(由申请人提供) 细胞组织化学核心为DERC附属研究人员所需的组织化学和形态方法提供最先进的技术支持、设施和协作专业知识。细胞组织化学核心服务和设施包括组织固定、石蜡和冷冻组织处理、组织学和组织化学染色、免疫细胞化学、原位杂交、放射自显影、显微摄影和计算机成像、电子显微镜和形态定量分析。细胞组织化学核心主任Denis G.Baskin博士是组织化学和糖尿病/内分泌学研究领域的知名科学家。他是华盛顿大学的医学研究教授，也是退伍军人医学研究服务处的高级研究职业科学家。CORE主任与DERC附属调查人员合作，监督CORE的运作。为了回应DERC附属机构对电子显微镜核心支持的请求，细胞组织化学核心招募了托马斯·鬼魂博士担任副核心主任。鬼魂博士是血管生物学和细胞外基质领域的著名细胞生物学家，负责监督核心的电子显微镜服务，其中包括一名兼职技术人员。向核心用户评估按存储容量使用计费，以抵消核心服务的成本。细胞组织化学核心是一大批DERC调查人员的经济高效的资源，多年来成功地为附属调查人员的许多出版物做出了贡献。进入新的资助期，细胞组织化学核心中心收到了37名附属调查人员对40个项目的服务请求，所有这些项目都得到了同行审查的赠款支持。DERC还建议在细胞组织化学核心中增加一个激光捕获显微切割系统，使附属机构能够获得组织和细胞的微观样本，用于与糖尿病相关的分子和生化分析。

Description (Provided by applicant) The Cytohistochemistry Core provides state-of-the-art technical support, facilities, and collaborative expertise for histochemical and morphological methods needed by DERC Affiliate Investigators. Cytohistochemistry Core services and facilities include tissue fixation, paraffin and cryostat tissue processing, histological and histochemical staining, immunocytochemistry, in situ hybridization, autoradiography, photomicrography and computer imaging, electron microscopy, and quantitative morphometric analysis. The Cytohistochemistry Core Director, Dr. Denis G. Baskin, is an established scientist in the fields of histochemistry and diabetes/endocrinology research. He is Research Professor of Medicine at the University of Washington and is also Senior Research Career Scientist in the VA Medical Research Service. The Core Director collaborates with DERC Affiliate Investigators and oversees the operations of the Core. In response to DERC Affiliate requests for electron microscopy Core support, the Cytohistochemistry Core recruited Dr. Thomas Wight to the position of Associate Core Director. Dr. Wight, a prominent cell biologist in the area of vascular biology and extracellular matrix, oversees the electron microscopy services of the Core, including a part-time technician. Charge backs are assessed to Core users to offset the cost of Core services. The Cytohistochemistry Core is a cost-efficient resource for a large group of DERC investigators and has been successful over the years in contributing to numerous publications by Affiliate Investigators. Going into the new funding period, the Cytohistochemistry Core has requests for service by 37 Affiliate Investigators for 40 projects, all with peer-reviewed grant support. The DERC also proposes to add a laser capture microdissection system to the Cytohistochemistry Core to provide Affiliates with the capability of obtaining microscopic samples of tissues and cells for molecular and biochemical analysis related to diabetes.