Cytokinesis and RNA Segregation in Zebrafish Development

胞质分裂是使子细胞分裂的物理过程，对发育至关重要。 和增长调控。在许多情况下，胞质分裂与不对称分离相结合。 细胞决定因素，进而导致细胞类型的功能多样化。中的一个基本问题 生物学是为了了解细胞命运决定因素分离的分子机制，在 特别是生殖质，一种特殊的细胞质，决定生殖细胞的命运。系统筛选 斑马鱼Danio rerio的母系效应突变已经确定了影响 胞质分裂和种质分离，在这种有机体中是紧密相连的。基因气场 似乎参与了细胞分裂和种质所需的膜和/或f-肌动蛋白动力学。 募集，细胞岛，可能编码染色体乘客蛋白极光B激酶，是 参与了沟渠的萌芽。编码L-天冬氨酸脱氢酶的基因nebel是很重要的 用于放大与皱纹成熟和种质紧凑相关的钙波。在……里面 此外，我们已经确定了非肌肉肌球蛋白II在皱纹成熟和种质中的作用。 种族隔离。 拟议中的研究将确定这些基因和其他亚细胞事件的确切作用，如 随着细胞骨架重排、皮质转运和膜胞吐作用的协调， 驱动细胞质分裂和种质分离的事件途径。对这些的理解 流程将提供对各种鲜为人知的主题的见解：i)涉及的事件 胞质分裂，ii)分裂细胞内种质成分的不对称分离，以及iii) 这两个过程所需的细胞机制之间的功能关系。

Cytokinesis, the physical process that divides daughter cells, is of fundamental importance to development and growth regulation. In many instances, cytokinesis is coupled with the asymmetric segregation of cellular determinants, which in turn results in the functional diversification of cell types. A basic question in biology is to understand the molecular mechanisms underlying the segregation of cell fate determinants, in particular the germ plasm, a specialized cytoplasm that confers the germ cell fate. Systematic screens for maternal-effect mutations in the zebrafish, Danio rerio, have identified genes that affect the processes of cytokinesis and germ plasm segregation, which in this organism are intimately linked. The gene aura appears to be involved in membrane and/or f-actin dynamics required for cell division and germ plasm recruitment, cellular island, which likely codes for the chromosomal passenger protein aurora B kinase, is involved in furrow initiation. The gene nebel, which likely encodes L-aspartate dehydrogenase is important for the amplification of calcium waves associated with furrow maturation and germ plasm compaction. In addition, we have identified a role for non-muscle myosin II in furrow maturation and germ plasm segregation. The proposed research will determine the precise roles of these genes and other subcellular events, such as cytoskeletal rearrangements, cortical transport and membrane exocytosis, within the coordinated pathway of events that drives cytokinesis and germ plasm segregation. The understanding of these processes will provide insights on a variety of poorly understood subjects: i) the events involved in cytokinesis, ii) the asymmetric segregation of germ plasm components within dividing cells, and iii) the functional relationship between the cellular mechanisms required for these two processes.