BIOMECHANICAL FACTORS THAT REGULATE OUTFLOW FACILITY IN HUMAN EYES

描述（由申请人提供）：调节人眼流出功能的生物力学因素。眼压升高是青光眼视神经损害的主要危险因素。本研究中待检验的中心假设是IOP的周期性变化影响影响通过常规流出途径的液体流动的调节机制。实验将使用前节灌注模型，使用死后人眼进行。具体目的如下：1）研究眼房水分泌的昼夜波动影响常规流出功能的机制。2)研究脉动性眼内压如何影响调节传统流出设施的机制。为了这两个目的，对照组和实验组将随着时间的推移进行比较，以观察这些生理应激源如何影响以下内孔：-流出设施/IOP，-离体小梁网细胞的mRNA水平，-常规流出细胞的蛋白质分泌，以及-施累姆氏管内壁中的孔密度。在这个建议中概述的实验将检查这两个生理相关的机械应力对眼前节灌注的生理、形态和生化影响

DESCRIPTION (provided by applicant): Biomechanical factors that regulate outflow facility in human eyes. Elevated intraocular pressure (IOP) is the primary risk factor for optic nerve damage in glaucoma. The central hypothesis to be tested within this research is that periodic changes in IOP impact regulatory mechanisms that affect fluid flow through the conventional outflow pathway. Experiments will be performed using the anterior segment perfusion model using post mortem human eyes. The specific aims are as follows: 1) To examine mechanisms by which diurnal oscillations in aqueous secretion affect conventional outflow facility. 2) To investigate how pulsatile intraocular pressure influences mechanisms that regulate conventional outflow facility. For both aims, control and experimental groups will be compared over time, to observe how these physiological stressors affect the following endopints: - Outflow facility/lOP, - mRNA levels of trabecular meshwork cells ex-vivo, - protein secretion by conventional outflow cells, and - pore density in the inner wall of Schlemm's canal. The experiments outlined in this proposal will examine the physiological, morphological, and biochemical effects of these two physiologically relevant mechanical stressors on the anterior segment perfusion