Cell Therapy Using Stem Cells and Biomimetic Biodegradeable Nanostructured Materials

在该项目的最后一年，将生成数据，以支持与用于生产用于骨再生的多孔生物活性玻璃支架的创新制造工艺相关的新知识产权申请。生物活性玻璃具有骨诱导的潜力，但目前可用的多孔生物活性玻璃产品很少。初步结果表明，在这个项目中开发的多孔支架可能有潜力超过许多市售的骨空隙填充物。聚谷氨酸已被成功地修饰和静电纺丝，所得纤维具有良好的细胞附着、活力和分化支持。在该项目完成后，所产生的数据很可能将允许开发双相骨软骨塞。该项目使Plasticell有限公司能够开发出从间充质干细胞生成软骨细胞和成骨细胞的新分化方案，从而进入干细胞培养试剂市场。该方案与目前可用的产品不同，因为它们不含血清和动物成分，因此使其更具可重复性，更适合临床应用。

In the final year of this project, data will be generated to support a new IP filing related to an innovative manufacturing process used to produce a porous bioactive glass scaffold for bone regeneration. Bioactive glass has the potential to be osteoinductive but there are currently very few porous bioactive glass products available. Preliminary results have shown that the porous scaffold developed in this project may have the potential to outperform many commercially available bone void fillers. Poly-y-glutamic acid has been successfully modified and electrospun with the resultant fibres showing good support of cell attachment, viability and differentiation. Following completion of this project, it is likely that the data generated will allow a biphasic osteochondral plug to be developed. The project has allowed Plasticell limited to develop novel differentiation protocols for the generation of chondrocytes and osteoblasts from mesenchymal stem cells, and thus enter the stem cell culture reagents market. The protocols differ from currently available products as they are serum-free and animal component free, thus making them more reproducible and suitable for clinical application.