CORE--MORPHOLOGY, IMMUNOLOGY AND MOLECULAR BIOLOGY

形态、免疫学和分子生物学核心将提供该计划中的各种项目 拥有必要的专业知识、设施和支持来执行常规光学显微镜、免疫荧光、 显微镜等常规和专业的形态、病理和免疫组织化学 需要支持 核心的分子生物学部分将包括DNA、蛋白质和 重组病毒；cDNA定点突变，转基因载体和质粒的生产， 通道蛋白在哺乳动物细胞培养中的表达与分析。核心也将是主要的 转基因小鼠群体的生产和维护设施。这些责任将 包括通过免疫化学手段进行育种、基因分型和表型分析。

The Morphology, Immunology and Molecular Biology Core will provide the various projects in the program with the necessary expertise, facilities, and support to perform routing light microscopy, immunofluorescent, microscopy, and other routine and specialized morphological, pathological and immunohistochemical support required The Molecular Biology component of the Core will include the growth and purification of DNA, proteins and recombinant viruses; site directed mutagenesis of cDNA, production of transfection vectors and plasmids, expression and analysis of channel proteins in mammalian cell cultures. The core will also be the primary facility for the production and maintenance of the transgenic mouse colonies. These responsibilities will include breeding, genotyping and phenotypic analysis by immunochemical means.