Mouse Modeling Core

介绍修订后的核心C：我们非常感谢评审者的积极评价 关于鼠标建模核心设施。如下所述，我们对提出的问题作出了答复。 并希望引起人们对我们核心服务的一个重要补充的注意。我们最近有过 描述了一种新的实验性子宫内膜异位症模型，使用的是Rag2y(C)小鼠(Bruner-Tran等人，2006b)。到期 对于这种免疫抑制小鼠品系的免疫缺陷的严重性，我们已经能够 将人类免疫细胞和子宫内膜细胞移植到这只动物身上，以便检查其潜力 免疫细胞在子宫内膜异位症病理生理中的作用虽然这种模式还没有完全实现 我们预计在未来12个月内完成这些研究，并制造出这个模型 之后立即提供给中心调查人员。我们相信这一模式系统将是一种有价值的 除了鼠标建模核心，它应该有助于我们理解开发和 治疗子宫内膜异位症。？ 1.过去的经验或描述对装运成功的嫁接进行评估的计划 没有对样品进行讨论。这些信息现已包含在核心描述中。 具体地说，Core实验室之前曾检查过隔夜运送新鲜组织的影响 植入老鼠体内，我们没有发现病变的大小和数量之间有明显的差异 与未发货的纸巾相比。此外，我们之前已经证明了组织 可预培养长达48小时(而不是典型的24小时)，对细胞存活或 病变植入率进一步支持组织连夜运输然后培养的可行性 24小时后进入小鼠体内。 2.耶鲁大学的PLS和核心实验室之间的沟通将是至关重要的，应该是 更详细地讨论了。正如核心描述中现在详细描述的那样，奥斯汀博士和布鲁纳-特朗博士 对于我们的合作者来说，在“非现场”地点进行研究有丰富的经验。我们预见到 与耶鲁大学的调查人员合作没有任何困难。 3.很少讨论内部和外部审查小组在以下方面的作用 对核心的监督。外部审查将每年进行两次，期间交替进行 范德比尔特和耶鲁。耶鲁大学的内部审查小组还将访问范德比尔特网站(The Mouse CORE和奥斯汀博士的实验室)。 突出显示更改/更正：本应用程序中显著更改的部分 以前提交的材料在案文左侧有一条粗线条，如下所示 呈现在本段的左侧。

Introduction to Revised Core C: We greatly appreciate the positive comments of the reviewers regarding the Mouse Modeling Core facility. We have responded to the issues raised, as outlined below, and would also like to draw attention an important addition to our Core services. We have very recently described a new experimental endometriosis model, using Rag2y(c) mice (Bruner-Tran et al, 2006b). Due to the severity of the immune defects of this immunosuppressed mouse strain, we have been able to transfer both human immune and endometrial cells to this animal, allowing examination of the potential role of immune cells in the pathophysiology of endometriosis. Although this model has not yet been fully characterized, we anticipate completing these studies within the next 12 months and making this model available to Center investigators immediately thereafter. We believe this model system will be a valuable addition to the Mouse Modeling Core which should aid our understanding both the development and treatment of endometriosis. ¿ 1. Past experience or a plan describing assessment of successful engraftment of shipped samples was not discussed. This information has now been included within the Core description. Specifically, the Core lab has previously examined the effect of overnight shipment of fresh tissues prior to engraftment into mice and we have found no noticeable difference between size or number of lesions compared to tissues that were not shipped. Additionally, we have previously demonstrated that tissues can be pre-cultured for up to 48 hrs (versus the typical 24 hrs) without noticeable impact on cell survival or lesion implantation rate further supporting the feasibility of shipping tissues overnight and then culturing 24 hrs prior to introduction into mice. 2. Communication between the PLs at Yale and the Core lab will be critical and should be discussed in more detail. As now detailed within the Core description, Drs. Osteen and Bruner-Tran have extensive experience conducting studies as an "off-site" location for our collaborators. We foresee no difficulties in working with investigators at Yale. 3. There was little discussion regarding the role of internal and external review groups in oversight of the Core. External review will be conducted twice each year, alternating between Vanderbilt and Yale. Yale's internal review group will additionally visit the Vanderbilt sites (the Mouse Core and Dr. Osteen's laboratory) at regular intervals. Highlighting of Changes/Corrections: Sections of this application which are significantly altered from the previous submission have been indicated by the presence of a bold line to the left of the text, as present to the left of this paragraph.