Robust Self-Assembled Monolayers for Improved Hemocompatibility

描述(由申请人提供)：用于改善血液相容性的坚固的自组装单分子膜首席调查者：Brink，Damon项目摘要/摘要：可植入血液接触血液的医疗设备，如左心室辅助设备(VAD)会给患者带来血栓风险。这些风险在儿科脑室辅助装置的细小血液管道中加剧。虽然商业上可用的血液相容性涂层还不能提供完全惰性的表面来防止血栓形成，但最近的研究表明，聚乙二醇单分子层(SAM)在这方面很有希望。不幸的是，这些涂层与表面的结合很弱，脱附会迅速降低性能。该项目的目标是形成惰性、共价结合的聚乙二醇单分子膜，这种膜不会降解，并将延长VAD和其他可植入设备的使用寿命。这将使用一种基于超临界二氧化碳的新的SAM沉积方法来实现，该方法可产生具有高接枝密度和出色一致性的坚固的单分子膜。涂层将使用表面分析方法进行充分表征。蛋白质吸附和细胞黏附将在受控流动条件下使用全血分析来探测。用于改善血液相容性的坚固的自组装单分子膜首席研究员：Brink，Damon项目叙述血栓形成的风险在心室辅助设备中是重要的--特别是在儿科设备的小血液管道中。这项工作旨在通过在钛和氧化铝衬底上提供共价化学键来促进有前景的聚乙二醇涂层的发展。共价粘接将提高聚乙二醇涂层在流动诱导剪切载荷下的耐久性。当通过超临界二氧化碳溶液沉积时，聚乙二醇将与硅烷官能团相连并形成高度均匀的自组装单分子膜(SAM)。

DESCRIPTION (provided by applicant): Robust Self-Assembled Monolayers for Improved Hemocompatibility Principal Investigator: Brink, Damon Project Summary / Abstract Implantable blood-contacting medical devices such as left ventricular assist devices (VADs) pose thrombosis risks to patients. These risks are exacerbated in the small blood conduits of pediatric ventricular assist devices. While commercially available hemocompatible coatings do not yet provide fully inert surfaces that resist clot formation, recent research suggests that self-assembled monolayers (SAMs) of poly(ethylene glycol) (PEG) hold promise in this regard. Unfortunately, these coatings are only weakly bonded to the surface and desorption quickly degrades performance. The aim of this project is to form inert, covalently-bonded PEG monolayers that do not degrade and will extend the usable life of VADs and other implantable devices. This will be accomplished using a novel SAM deposition method based on supercritical carbon dioxide which produces robust monolayers having high graft density and outstanding uniformity. The coatings will be fully characterized using surface analysis methods. Protein adsorption and cellular adhesion will be probed using whole blood assays under controlled flow conditions. Robust Self-Assembled Monolayers for Improved Hemocompatibility Principal Investigator: Brink, Damon Project Narrative Risks of thrombosis formation are significant in ventricular assist devices - particularly in the small blood conduits of pediatric devices. This work seeks to advance the promising poly(ethylene glycol) (PEG) coatings by providing a covalent chemical bond to titanium and alumina substrates. Covalent bonding will enhance the durability of the PEG coatings in the presence of flow induced shear loads. The PEG will be linked with silane functional groups and form a highly uniform self-assembled monolayer (SAM) when deposited via a solution of supercritical carbon dioxide.