Adherence Assessment for Supplements

描述(由申请人提供)：在NCCAM临床试验中，Caldera制药公司使用蛋白质分级技术和XRPro技术来确定蛋白质CAM/代谢物结合，作为确定遵守CAM方案的一种手段。Caldera将使用他们的高通量蛋白质组学技术来识别CAM依从性生物标记物(CAB)，该标记物使用我们独特的、在全国获奖的XRpro技术。火山口与洛斯阿拉莫斯国家实验室合作开发了XRPro。这项技术允许通过测量由蛋白质-配体加合物和蛋白质-金属代谢物组成的CAB来识别和测量CAM粘附性，而不需要事先知道涉及哪些蛋白质。因此，不需要针对特定蛋白质的抗体或特定蛋白质检测。Caldera制药公司拥有2000平方英尺的实验室空间和1800平方英尺的办公空间。这一地区进行化学和生物研发的设备齐全，包括：通风柜、灭菌炉、反应玻璃器皿、旋转蒸发器、反应和蒸馏真空线、气氛控制手套箱、加热/搅拌套筒、光谱仪(UV-VIS-NIR)、核磁共振、结晶学、荧光仪、FTIR、TGA和DSC、显微镜、定制的EDAX Eagle III MXRF光谱仪和定制的EDAX Eagle II MXRF光谱仪和Horiba XGT 5000 XRF光谱仪，以及定制的共焦XRF显微镜、FPLC和应用生物系统Voyager-STR MADE LDI-TOF和QSTAR/MS质谱仪。火山口可以进入机械和电子产品商店。火山口设施符合联邦、州和地方政府关于以下方面的环境法律和法规：空气排放、水上流出物、外部辐射水平、室外噪音、固体和散装废物处理做法以及有毒和危险材料的处理和储存。

DESCRIPTION (provided by applicant): Caldera Pharmaceuticals uses protein fractionation techniques and XRpro technology to determine protein CAM/metabolite binding as a means to determine adherence to CAM regimens in NCCAM clinical trials. Caldera will use their high-throughput proteomics technology to identify CAM Adherence Biomarkers (CABs) using our unique, national award winning XRpro technology. Caldera, in collaboration with Los Alamos National Laboratory, developed XRpro. This technology allows the identification and measurement of CAM adherence by measuring CABs consisting of protein-ligand adducts and protein-metal metabolites with no a priori knowledge of which proteins are involved. Therefore, antibodies to specific proteins or specific protein assays are not required. Caldera Pharmaceuticals has 2000 square feet of lab space and 1800 square feet of office space. This area is well equipped for chemical and biological R&D. Equipment available for this work includes: fume hoods, sterilization ovens, reaction glassware, rotary evaporator, vacuum lines for reactions and distillations, atmosphere controlled glove boxes, heating/stirring mantles, spectrometers (UV-VIS-NIR), NMR, crystallography, fluorimeters, FTIR, TGA and DSC, microscopes, a customized EDAX Eagle III MXRF spectrometer and a customized EDAX Eagle II MXRF spectrometer and Horiba XGT 5000 XRF microscope, and a custom-built confocal XRF microscope, FPLC, and an Applied Biosystems Voyager-DE STR MALDI-TOF and QSTAR XL MS/MS mass spectrometer. Caldera has access to machine and electronics shops. Caldera facilities meet environmental laws and regulations of federal, state, and local governments relating to: airborne emissions, waterborne effluents, external radiation levels, outdoor noise, solid and bulk waste disposal practices, and handling and storage of toxic and hazardous materials.