MODULE--TISSUE AND CELL CULTURE

该单元的目标是：(1)协助研究人员准备原代细胞培养 从眼组织中提取；(2)维持已建立的细胞系；(3)帮助制造特殊的 用于组织维护的密室。该模块旨在满足我们的基本需求 研究人员获取视网膜和角膜细胞的细胞培养。细胞和组织培养已经被 为研究复杂现象提供简单的系统至关重要。它们的优点是：(1)细胞在 培养物可长时间繁殖；(2)它们提供同质的制剂 对于实验和(3)更容易操纵他们的环境。 细胞培养系统有一定的缺陷。例如，它们需要相当高的技能。 在组织解剖中，使周围组织的污染降至最低。细胞可以具有 特殊的生长要求，寻找合适的细胞培养条件是一项耗时的工作 这项任务艰巨而艰巨。由于这些限制，调查人员往往不愿尝试在 研究生理问题的体外组织和细胞培养系统。纸巾的可用性和 细胞培养核心设施和熟练研究技术人员的专家帮助应有助于 更多关于眼睛组织的探索性研究。此外，该模块还将帮助存储和维护 已建立的眼部细胞系。

The goals of the module are: (1) to assist investigators in the preparation of primary cell cultures from ocular tissues; (2) to maintain established cell lines and (3) help in the fabrication of special chambers for tissue maintenance. The module aims to fulfill the fundamental needs of our investigators to obtain cell cultures of retinal and corneal cells. Cell and tissue cultures have been crucial providing simple systems for studying complex phenomenon. Their strengths are: (1) cells in culture can be propagated for extended periods of time; ((2) they provide homogenous preparations for experimentation and (3) it is easier to manipulate their environment. The cell cultures systems have certain drawbacks. For example, they require considerable skill in tissue dissection, so that there is minimal contamination from surrounding tissues. Cells can have peculiar growth requirements, and finding the proper conditions for cell culture is a time-consuming and laborious task. Because of these limitations, investigators are often reluctant to try to establish in vitro tissue and cell culture systems to study physiological problems. The availability of a tissue and cell culture core facility along with the expert help from a skilled research technician should facilitate more exploratory research with eye tissues. In addition, the module will help to store and maintain established, ocular cell lines.