800 MHz NMR Spectrometer for High-End NMR Structural Studies

描述（由申请人提供）：弗吉尼亚大学的八名教师正在使用高分辨率多维核磁共振进行生物聚合物的研究。这些研究人员都得到了来自美国国立卫生研究院和其他来源的资助。利用核磁共振来阐明生物分子的结构和功能是他们NIH资助的研究的一个组成部分。由于这些研究人员正在研究的系统面临的挑战，包括溶解度和/或稳定性的限制，缺乏分散（扩散），以及对大型系统（如在洗涤剂中溶解的整体膜蛋白）的关注，该小组已经确定了对校园高场核磁共振仪器的独特需求。为此，我们正在为一台18.8 T （800 MHz 1H）核磁共振（NMR）光谱仪申请资金，该光谱仪将位于弗吉尼亚大学。该光谱仪将用于高分辨率的多维核磁共振实验，标记（15N， 13C和15N， 13C, 2H）生物聚合物在溶液中，以及在各种对准介质的存在。为了适应安装场地的要求，磁体将被主动屏蔽，以尽量减少杂散场对邻近实验室的影响。该光谱仪将配备4个射频通道，其中一个双全频段射频系统能够在1H频率及以下工作，另一个双宽带射频系统能够在15N至31P频率范围内工作。该光谱仪还将配备配备单轴脉冲场梯度能力的氦冷却三共振h探测低温探针。这种探头在信噪比方面提供了显著的改进，这对于大多数用户应用来说是必不可少的。800 MHz核磁共振波谱仪将在三个关键性能标准上提供显着增强，这对我们用户组的研究工作至关重要：1)800 MHz核磁共振波谱仪提供了信噪比（S/N）的大幅增加。2) 800 MHz核磁共振波谱仪大幅度增加了核磁共振信号的色散（扩散）。3) 800 MHz核磁共振波谱仪提高了基于trosy的实验性能。

DESCRIPTION (provided by applicant): Eight faculty members at the University of Virginia are using high-resolution multi-dimensional NMR in their studies of biopolymers. These investigators are all well-funded with grants from the NIH and other sources. The use of NMR to elucidate biomolecular structure and function is an integral part of their NIH funded research. Due to the challenges of the systems being worked on by these investigators, including limitations in solubility and/or stability, lack of dispersion (spread), and a focus on large systems such as integral membrane proteins solubilized in detergents, this group has identified a distinct need for higher field NMR instrumentation on campus. To this end, we are requesting funding for an 18.8 T (800 MHz 1H) nuclear magnetic resonance (NMR) spectrometer to be sited at the University of Virginia. This spectrometer will be used for high-resolution multi- dimensional NMR experiments with labeled (15N, 13C and 15N, 13C, 2H) biopolymers in solution as well as in the presence of various alignment media. In order to accommodate the requirements of the installation site, the magnet will be actively shielded to minimize the effects of the stray field on neighboring laboratories. The spectrometer will be equipped with 4 radiofrequency (RF) channels with one dual fullband RF system capable of operating at the 1H frequency and lower and one dual broadband RF system capable of operating at the 15N to 31P frequency range. The spectrometer will also be equipped with a He-cooled triple resonance 1H-detection cryoprobe equipped with a single-axis pulsed-field gradient capability. This probe provides a dramatic improvement in the signal-to-noise which is essential for the majority of the user applications. The 800 MHz NMR spectrometer will provide dramatic enhancements in three critical performance criteria that are essential for the research efforts of our user group: 1) The 800 MHz NMR spectrometer provides a substantial increase in the signal-to-noise (S/N). 2) The 800 MHz NMR spectrometer provides a substantial increase in the dispersion (spread) of NMR signals. 3) The 800 MHz NMR spectrometer enhances the performance of TROSY-based experiments.