PURCHASE OF A XENOGEN'S IVIS IMAGING SYSTEM 200 WORKSTATION: INFECTIOUS DISEASE

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。此共享仪器拨款申请要求支持购买Xenogen双光子工作站，用于整个小动物的成像。该仪器将集成到现有的Englert图像分析设备中，该设备是达特茅斯医学院诺里斯棉花癌症中心的共享资源。该工作站将为达特茅斯学院、塞耶工程学院、达特茅斯医学院和达特茅斯-希区柯克医疗中心的美国国立卫生研究院资助的科学家社区提供新技术。作为在达特茅斯扩大全动物成像的全面努力的一部分，我们建议购买Xenogen IVIS“成像系统”。该工作站将允许对小动物的细胞和生物制品进行光学（荧光/生物发光）成像，并允许实时成像癌细胞的迁移、治疗药物的生物分布、药代动力学研究和基因原位表达研究。这项新技术将使达特茅斯学院、美国国立卫生研究院资助的科学家们能够解决现有的、可用的技术迄今无法解决的具体问题。此外，由于动物可以连续评估肿瘤生长或基因表达，这项新技术将大大减少用于实验的动物数量。Englert图像分析设施是一个已建立的共享资源，由许多机构资源支持，由主任、联合主任和咨询委员会指导。学院拥有广泛的科学和技术专业知识，可以有效地开发光学成像技术。来自塞耶工程学院（Thayer School of Engineering）的全动物成像和分析专家将成为达特茅斯这项技术发展的重要组成部分。Xenogen工作站的长期支持和维护将由NCI向Norris Cotton癌症中心提供的核心资助，以及达特茅斯医学院的用户费用和机构支持来满足。此外，达特茅斯医学院已承诺出资5万美元购买这台仪器。该仪器的集成将满足在达特茅斯建立整个动物成像技术的总体任务中一个重要的、必要的组成部分。我们已经确定了12个主要用户和2个次要用户，鉴于Xenogen工作站的可用性，他们的研究能力将得到显著扩展。这项新技术的作用是满足美国国立卫生研究院资助的这些研究项目的具体目标的需要。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. This Shared Instrumentation Grant application requests support to purchase a Xenogen biphotonic workstation for the imaging of whole, small animals. The instrument will be integrated into the existing Englert Image Analysis Facility, which is a shared resource of the Norris Cotton Cancer Center at Dartmouth Medical School. The workstation will provide new technologies to the community of NIH-funded scientists at Dartmouth College, the Thayer School of Engineering, Dartmouth Medical School and Dartmouth-Hitchcock Medical Center. As part of a comprehensive effort to expand whole animal imaging at Dartmouth, we propose the purchase of a Xenogen IVIS" Imaging System. This workstation will permit optical (fluorescence/bioluminescence) imaging of cells and biologicals in small animals and allow to image, in real time, the migration of cancer cells, the biodistribution of therapeutics, pharmacokinetic studies and studies of gene expression in situ. This new technology will allow Dartmouth, NIH-funded scientists to address specific questions that are heretofore unapproachable given existing, available technologies. In addition, because animals can be serially assessed for tumor growth or gene expression, this new technology will greatly reduce the number of animals needed for use in experimentation. The Englert Image Analysis Facility is an established shared resource supported by a number of institutional sources and is directed by a Director, co-director and an Advisory Board. There is extensive scientific and technical expertise at the faculty level for effective development of optical imaging technology. Faculty from the Thayer School of Engineering, who are experts in whole animal imaging and analysis will be an integral part of the development of this technology at Dartmouth. Long-term support and maintenance of the Xenogen workstation will be met by support from the NCI Core Grant to the Norris Cotton Cancer Center, together with users fees as well as institutional support from Dartmouth Medical School. Furthermore, Dartmouth Medical School has committed $50,000 towards the purchase of this instrument. The integration of this instrument will meet an important, needed component in the overall mission to build whole animal imaging technology at Dartmouth. We have identified 12 major and 2 minor users whose research capabilities will be markedly expanded given the availability of the Xenogen workstation. The role of this new technology to meet the needs of the NIH-sponsored specific aims for each of these research programs is presented.