PEPTIDE SYNTHESIS AND ANALYSIS EQUIPMENT: ALZHEIMER'S, PARKINSON'S, FRAGILE X

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：申请支持购买自动化固相肽合成和分析设备。该设备包括一台应用生物系统433A型大型多肽合成仪和一台Waters Model Alliance分析高效液相色谱仪，用于评估合成多肽的纯度。这些仪器在操作上是一个集成的系统，对于埃默里大学微量化学和蛋白质组学设施生产多肽是必不可少的。由于需要更换已有18年历史的大型合成器(应用生物系统430A)和已有18年历史的分析型高效液相色谱仪(应用生物系统120A)，因此有理由要求购买这些仪器，这些设备已不再受制造商支持。主要受益者将是NIH支持的29名研究人员的主要用户组，他们隶属于13个基础和临床科学部门。特别强调的是：1)合成高纯度的修饰多肽，包括磷酸多肽、脂肪酸修饰多肽和具有治疗意义的多肽类似物，包括抗菌肽；2)在支持的研究项目中协助多肽的所有方面的使用；3)培训研究生和博士后研究员。另外49名由NIH支持的埃默里调查人员也将受益于使用这种设备。除了多肽合成，该设施还提供核酸合成、多肽和蛋白质质谱仪以及其他生物分析和蛋白质组学服务，包括埃德曼测序、氨基酸分析和微分离。所要求的仪器将显著提高所述研究项目以及国家卫生研究院支持的其他项目和埃默里研究中心的生产率。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Support is requested for the purchase of automated solid-phase peptide synthesis and analysis equipment. This equipment includes an Applied Biosystems model 433A large-scale peptide synthesizer and a Waters model Alliance analytical HPLC to evaluate the purity of the synthesized peptides. These instruments are operationally an integrated system that is essential for producing peptides at the Emory University Microchemical and Proteomics Facility. The request for these instruments is justified by the need to replace an eighteen-year-old large-scale synthesizer (Applied Biosystems 430A) and an eighteen-year-old analytical HPLC (Applied Biosystems 120A), which are no longer supported by the manufacturer. The major beneficiaries will be the Major User Group of 29 NIH-supported investigators who are affiliated with 13 basic and clinical science departments. A special emphasis is on 1) synthesis of highly purified modified peptides including phosphopeptides, fatty acid-modified peptides and peptide analogs of therapeutic interest including antibacterial peptides; 2) assistance with all aspects of peptide use in research projects supported; 3) training of graduate students and post-doctoral fellows. An additional 49 NIH-supported Emory investigators will also benefit from access to this equipment. In addition to peptide synthesis, the Facility provides nucleic acid synthesis, peptide and protein mass spectrometry, and other bioanalytical and proteomics services including Edman sequencing, amino acid analysis, and micro-separations. The requested instrumentation will significantly enhance the productivity of the research projects described as well as that of other NIH supported projects and research centers at Emory.