PEPTIDE SYNTHESIS AND ANALYSIS EQUIPMENT: PROTEOMICS

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：要求支持购买自动化固相肽合成和分析设备。该设备包括Applied Biosystems型号433 A大规模肽合成仪和沃茨型号Alliance分析HPLC，以评价合成肽的纯度。这些仪器在操作上是一个集成系统，对于埃默里大学微量化学和蛋白质组学设施的肽生产至关重要。要求提供这些仪器的理由是，需要更换一台已有18年历史的大型合成仪（Applied Biosystems 430 A）和一台已有18年历史的分析型HPLC（Applied Biosystems 120 A），这些仪器已不再得到制造商的支持。主要受益者将是隶属于13个基础和临床科学部门的29名NIH支持的研究人员的主要用户组。特别强调的是1）合成高纯度的修饰肽，包括磷酸肽，脂肪酸修饰肽和具有治疗意义的肽类似物，包括抗菌肽; 2）在支持的研究项目中协助肽的所有方面; 3）培训研究生和博士后研究员。另外49名NIH支持的埃默里研究人员也将受益于使用该设备。除了肽合成，该设施还提供核酸合成，肽和蛋白质质谱，以及其他生物分析和蛋白质组学服务，包括Edman测序，氨基酸分析和微量分离。所要求的仪器将显着提高所描述的研究项目以及其他NIH支持的项目和埃默里大学研究中心的生产力。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Support is requested for the purchase of automated solid-phase peptide synthesis and analysis equipment. This equipment includes an Applied Biosystems model 433A large-scale peptide synthesizer and a Waters model Alliance analytical HPLC to evaluate the purity of the synthesized peptides. These instruments are operationally an integrated system that is essential for producing peptides at the Emory University Microchemical and Proteomics Facility. The request for these instruments is justified by the need to replace an eighteen-year-old large-scale synthesizer (Applied Biosystems 430A) and an eighteen-year-old analytical HPLC (Applied Biosystems 120A), which are no longer supported by the manufacturer. The major beneficiaries will be the Major User Group of 29 NIH-supported investigators who are affiliated with 13 basic and clinical science departments. A special emphasis is on 1) synthesis of highly purified modified peptides including phosphopeptides, fatty acid-modified peptides and peptide analogs of therapeutic interest including antibacterial peptides; 2) assistance with all aspects of peptide use in research projects supported; 3) training of graduate students and post-doctoral fellows. An additional 49 NIH-supported Emory investigators will also benefit from access to this equipment. In addition to peptide synthesis, the Facility provides nucleic acid synthesis, peptide and protein mass spectrometry, and other bioanalytical and proteomics services including Edman sequencing, amino acid analysis, and micro-separations. The requested instrumentation will significantly enhance the productivity of the research projects described as well as that of other NIH supported projects and research centers at Emory.