AMERSHAM BIOSCIENCES TYPHOON 9410: DRUG ABUSE

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：此请求是购买Amersham Biosciences台风9410。“台风”将作为细胞生物学、微生物学和药理学部门的核心仪器，由29名研究人员组成的用户组，几乎都是由美国国立卫生研究院资助的，研究兴趣广泛。此外，纽约大学医学院内任何其他需要使用台风特殊功能的研究者将被允许加入用户组。目前在整个医学院都没有类似的仪器。台风9410在一台机器中结合了一个磷光成像仪、一个荧光图像扫描仪和一个发光读取器。除了Amersham以外，没有其他公司销售具有相同功能的金融工具。此外，台风将取代目前使用的过时的分子动力学425磷成像仪。由于用户组中的大多数实验室已经拥有分子动力学磁带，这些磁带只能由Amersham仪器读取，因此购买Typhoon是合适的且具有成本效益。“台风”还将取代目前位于另一栋建筑内的老化且有限的分子动力学荧光仪575。台风荧光仪比其前身更敏感，提供更好的分辨率，并可以检测更广泛的荧光基材。台风的检测能力不仅将提供改进的荧光测量，还将实现荧光探针标记的Western和核酸杂交斑点的定量成像，使用增强的化学发光代替x射线胶片检测斑点，并允许在一维和二维聚丙烯酰胺凝胶上用荧光染料染色的蛋白质的可视化和定量。还要求为软件许可证提供资金，以便在安装仪器的设施以外的各个部门设立数据分析工作站。用户组的一个小委员会将管理该工具并为其设计使用规则。一名训练有素的技术人员将对机器进行日常维护和监控，并对新用户进行培训。预计台风的可用性将对用户群体和整个机构的研究产生重大影响。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This request is for the purchase of an Amersham Biosciences Typhoon 9410. The Typhoon will serve as a core instrument for the Departments of Cell Biology, Microbiology, and Pharmacology with a user group of 29 investigators, nearly all with NIH funding, with a wide variety of research interests. In addition, any other investigator within the NYU School of Medicine who requires the use of the special capabilities of the Typhoon will be allowed to join the user group. No similar instrument currently exists in the entire School of Medicine. The Typhoon 9410 combines a phosphorimager, a fluorescence image scanner, and a luminescence reader in one machine. No company other than Amersham markets an instrument with the same capabilities. Moreover, the Typhoon will replace an obsolete Molecular Dynamics 425 phosphorimager currently in use. Since most of the laboratories in the user group already own Molecular Dynamics cassettes, which can only be read by the Amersham instrument, the purchase of the Typhoon is appropriate and cost effective. The Typhoon will also supplant an aging and limited Molecular Dynamics Fluorimeter 575 that is currently housed in a separate building. The Typhoon fluorimeter is much more sensitive than its predecessor, offers better resolution, and can detect a wider variety of fluorescent substrates. The detection capabilities of the Typhoon will not only provide improved fluorimetric measurements but also will enable quantitative imaging of Western and nucleic acid hybridization blots labeled with fluorescent probes, replace X-ray film for detection of blots using enhanced chemiluminescence, and allow visualization and quantitation of proteins stained with fluorescent dyes on one- and two-dimensional polyacrylamide gels. Funds are also requested for a software license to set up workstations for data analysis in the individual departments separate from the facility housing the instrument. A small committee of the user group will administer the instrument and design rules for its use. A trained technician will maintain and monitor the machine on a daily basis and educate new users. It is anticipated that the availability of the Typhoon will have great impact on the research of the user group and the institution as a whole.