CONFOCAL EQUIPMENT FOR BIOMEDICAL & NANOMEDICINE: PHYSIOLOGY

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：这项提案要求资金购买具有FRET和TIRF显微能力的激光扫描共聚焦显微镜系统。该仪器将安装在密苏里大学道尔顿心血管研究中心(DCRC)的成像设施中。虽然有几种现代荧光显微镜可供一般使用，但我们在这个地点没有共焦显微镜。这所大学对使用单一共聚焦显微镜的收费要求很高，造成进行活细胞/组织成像实验所需的大时间段的短缺。鉴于我们实验的时间敏感性，以及将活细胞/组织运送到这个地点的困难，在DCRC的成像核心上增加一个共聚焦显微镜将极大地增强正在进行的NIH资助的各种研究项目。如本提案所述，成立了一个咨询委员会，以确定核心用户群体，确定DCRC的具体需求，并选择适当的工具来实现我们研究小组的目标。在广泛咨询和分析了各种竞争对手的共焦系统后，奥林巴斯FluoView 1000被确定为理想的系统。该系统可以被修改为将FRET和TIRF功能与膜片钳技术相结合，以满足研究人员的需要，这些研究人员应用创新的荧光显微镜和纳米技术方法来解决膜生理学中的核心问题，如胞吐/内吞、离子通道门控和受体介导的信号转导。此外，这个共聚焦系统还将支持第二组研究人员，他们研究谷氨酸和GABA受体的亚细胞定位以及参与血压调节的中央核团中神经元模式的产生。拟议的共焦显微镜的加入不仅将最大限度地发挥我们现有研究计划的影响，还将有助于招聘新的有才华的教职员工加入我们研究所已经成功的团队。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal requests funds to purchase a laser scanning confocal microscope system with FRET and TIRF microscopic capabilities. The instrument will be housed within the imaging facility of Dalton Cardiovascular Research Center (DCRC) at the University of Missouri. While several modern fluorescent microscopes are available for general use, we do not have a confocal microscope at this site. There is a high demand placed on the fee-for-service access to the single confocal microscope available at this university creating shortage of large time blocks necessary for conducting live cell/tissue imaging experiments. In view of the time- sensitive nature of our experiments and difficulty in transporting live cells/ tissue to this location, the addition of a confocal microscope to the imaging core at DCRC would greatly enhance a diverse array of ongoing NIH-funded research programs. As detailed in this proposal, an advisory committee was formed to identify a core group of users, determine the specific needs of DCRC and to choose the appropriate instrumentation to accomplish the goals of our research groups. Following extensive consultation and analysis of various competing confocal systems, the Olympus Fluoview 1000 has been identified as the ideal system. This system can be modified to integrate FRET and TIRF capabilities with patch-clamp techniques to serve the needs of investigators applying innovative fluorescent microscopy and nanotechnology approaches to central questions in membrane physiology such as exocytosis/ endocytosis, ion channel gating and receptor- mediated signal transduction. In addition, this confocal system will also support a second group of investigators studying subcellular localization of glutamate and GABA receptors and neuronal pattern generation in the central nuclei involved in blood pressure regulation. The addition of the proposed confocal microscope will not only maximize the impact of our existing research programs but also will help in recruiting new, talented faculty to join the already successful group at our Institution.