LSM 510 CONFOCAL MICROSCOPE: BRAIN

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(申请人提供)：此申请是为了获得购买蔡司510元共焦显微镜的资金。该乐器将放置在匹兹堡大学药学院索尔克厅的10楼。药学院的基础和临床研究在过去六年中有了巨大的增长。在过去的四年里，药学院一直被评为全国所有药学院中NIH资助的前十名。尽管取得了这一成功，但学校的设备相对较差，在许多情况下被迫依赖其他学校的主要设备。这不仅给现有人员造成了困难，也给招聘高素质的研究人员带来了障碍。这项请求的目的是为药学院提供自己的共焦仪器，以满足其研究人员的关键需求。具体地说，该学院目前需要一台四激光、光谱、共聚焦显微镜，配备用于活细胞成像。学校内六名由NIH资助的调查人员已经确定了对这种设备的需求。这些工作大多属于新药开发(特别是抗癌药物和基因治疗方法的开发)领域，重点是跟踪分子和药物在细胞和组织内的吸收和贩运。目前，药学院没有共焦仪器。仪器可以在大学内的其他地方获得，主要是在大学的生物成像中心(CBI)。不幸的是，CBI无法提供足够的通道来满足我们的需求。CBI的资源需求量非常大，药学学院的人员在获取方面的优先程度最低。例如，药学学院的工作人员在晚上或周末不能进入，如果有空位，只能有3个小时的时间段。通常情况下，药学院人员必须等待1-2周才能访问仪器，并且在访问期间无法分析和收集他们的所有数据。缺乏可用的仪器时间是非常令人沮丧的，并限制了我们的研究步伐。购买所需的仪器将消除这一问题，并将为药学学院提供急需的资源。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This request is for funds to purchase a Zeiss 510 meta confocal microscope. This instrument will be housed on the 10th floor of Salk Hall in the University of Pittsburgh School of Pharmacy. Basic and clinical research within the School of Pharmacy has grown tremendously over the past six years. During the past four years, the School of Pharmacy has been rated among the top 10 in NIH funding among all Schools of Pharmacy in the nation. Despite this success, the School is relatively equipment poor and is forced in many cases to rely on major equipment in other schools. Not only does this create difficulties for existing personnel, but also presents obstacles in recruiting highly qualified research faculty. The purpose of this request is to provide the School of Pharmacy with its own confocal instrument to meet the critical needs of its research faculty. Specifically, the School currently is in need of a four-laser, spectral, confocal microscope, equipped for live cell imaging. Six NIH-funded investigators within the School have identified a need for such equipment. Most of this work falls within the realm of new drug development (particularly the development of anti-cancer agents and gene therapy methodologies) and focuses on following the uptake and trafficking of molecules and drugs within cells and tissues. Currently, the School of Pharmacy has no confocal instrument. Instruments are available elsewhere within the University, primarily at the University's Center for Biologic Imaging (CBI). Unfortunately, CBI is not able to provide sufficient access to accommodate our needs. CBI resources are in very high demand, and School of Pharmacy personnel have the lowest priority with regard to access. For example, School of Pharmacy personnel have no access on evenings or weekends, and are limited to 3-hour time slots, when slots are available. Often School of Pharmacy personnel must wait 1-2 weeks before getting access to an instrument, and during the access time available are not able to analyze and collect all of their data. This lack of available instrument time is extremely frustrating and limits the pace of our research. Acquisition of the requested instrument will eliminate this problem, and will provide the School of Pharmacy with a greatly needed resource.