AUTOMATED HT REAL-TIME PCR SYSTEM: TRANSFORMING GENES IN MELANOMA

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：申请资金用于购买应用生物系统公司生产的自动化高通量实时PCR系统。7900 HT快速实时384/96孔（带有定制Zymark Twister Robot，用于自动上板）将位于Wistar Institute Genomics Facility。目前，威斯塔研究所还没有高通量自动化实时PCR的能力。也没有用电荷耦合器件（CCD）照相机测量多个荧光团的能力，其允许比传统PCR或其他真实的时间PCR仪器显著更高的精确度和准确度。这种准确度和精确度的提高对于单核苷酸多态性（SNP）的可靠高通量数据分析、微阵列RNA表达分析的定量验证以及全基因组染色质免疫沉淀分析的定量分析是必不可少的。该系统的独特功能将使8个主要用户受益，并为超过35个用户提供Wistar Genomics Core增强功能，这些用户占Wistar研究所的约50%，并大量使用宾夕法尼亚大学的基础和临床研究人员。该仪器的所有主要用户都得到了NIH的资助和需要增加阵列样本真实的PCR分析能力的项目。一个由Wistar研究所主要用户组成的咨询委员会将监督该工具的操作、维护和公平使用。该仪器将被集成到Wistar Genomics Core Facility中，该设施成立于1996年，致力于系统生物学计划的长期发展和基因组研究的技术开发。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Funds are requested for the purchase of an automated high-throughput real-time PCR system manufactured by Applied Biosystems. The 7900 HT Fast Real-Time 384/96 Well with custom Zymark Twister Robot for automatic plate loading capability will be located in the Wistar Institute Genomics Facility. There is currently no capability of high throughput automated real-time PCR available at the Wistar Institute. There is also no capability of measuring multiple fluorophores with a charge-coupled device (CCD) camera, which allows significantly higher precision and accuracy than traditional PCR or other real time PCR instruments. This increase in accuracy and precision is essential for reliable high throughput data analysis of single nucleotide polymorphisms (SNP), for quantitative verification of microarray RNA expression analysis, and for quantitative analysis of genome-wide chromatin immunoprecipitation analysis. The unique features of this system will benefit eight major users and provide the Wistar Genomics Core increased capabilities for over 35 users, representing ~50% of the Wistar Institute and substantial usage from basic and clinical investigators at the University of Pennsylvania. All major users of this instrument have NIH grant support and projects requiring increased capacity for real time PCR analysis of array samples. An advisory committee comprised of the major users from the Wistar Institute will oversee the operation, maintenance, and equitable access of this instrument. This instrument will be integrated in the Wistar Genomics Core Facility, which was established in 1996 and is committed to long-term development of systems biology program and technology development for genomic studies.