ChIP Sequencing Core

ChIP 测序核心

• 批准号: 8874170
• 负责人: PAUL M LIEBERMAN
• 金额: $ 12.55万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-08 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8874170
• 关键词: B-Lymphocytes; Bioinformatics; Biological Assay; ChIP-seq; DNA Sequencing Facility; Data; Data Analyses; Event; Genome; Genomics; Goals; Grant; Human Genome; Human Herpesvirus 4; Human Herpesvirus 8; Infection; Instruction; Lead; Libraries; Maps; Methods; Online Systems; Preparation; Production; Research Personnel; Sampling; Sequence Analysis; Services; The Wistar Institute; Time; Work; data management; follow-up; member; next generation; next generation sequencing; programs

PROJECT SUMMARY (See instructions): The GhlP-Seq Production core will be responsible for 1) performing ChlP-assays; 2) generating library for lllumina next-generation sequencing, 3) mapping sequence data to KSHV and human genomes, and 4) providing follow-up bioinformatics analyses. These goals will be. accomplished in conjunction with the Wistar Institutes Genomics and Bioinformatics Core, which provides lllumina Next Generation Sequencing as a core service. Final data management and analyses will be made accessible to each project investigator via a shared web-based browser for KSHV. Expertise in all aspects of ChIP, Next-Generation Sequencing, and Bioinformatic support will be provided to the projects for the lifetime of grant support. The GhlP-Seq core will be lead by Dr. Paul Lieberman, who is familler with ChIP Seq methods and bioinformatics analyses as applied to KSHV and EBV genomes. The ChlP-Seq core will facilitate the ability of all members of the Program project to standardize, methods for sample preparation and for post-sequence analysis. The work will be performed at the Wistar Institute in space within the Lieberman lab; and in conjunction with the Wistar Institute Genomics Core and Sequencing facility.

项目摘要（参见说明）：GhlP-Seq Production 核心将负责 1) 执行 ChlP 检测； 2) 生成用于 lllumina 下一代测序的文库，3) 将序列数据映射到 KSHV 和人类基因组，4) 提供后续生物信息学分析。这些目标将会实现。该项目是与 Wistar 研究所基因组学和生物信息学核心共同完成的，该核心提供 lllumina 下一代测序作为核心服务。每个项目调查员都可以通过 KSHV 的共享网络浏览器访问最终数据管理和分析。在资助期限内，将向项目提供 ChIP、下一代测序和生物信息学支持等各个方面的专业知识。 GhlP-Seq 核心将由 Paul Lieberman 博士领导，他熟悉应用于 KSHV 和 EBV 基因组的 ChIP Seq 方法和生物信息学分析。 ChlP-Seq 核心将促进该计划项目所有成员标准化样品制备和序列后分析方法的能力。这项工作将在利伯曼实验室内的威斯塔太空研究所进行；并与 Wistar 研究所基因组学核心和测序设施相结合。