Purchase of an Automated HT Real-Time PCR System

描述(申请人提供)：申请资金用于购买由应用生物系统公司制造的自动化高通量实时聚合酶链式反应系统。配备定制Zymark Twister Robot的7900 HT Fast-Real-Time 384/96 Well将安装在Wistar研究所基因组学设施中，以实现自动平板加载能力。维斯塔尔研究所目前还没有高通量自动化实时聚合酶链式反应的能力。此外，还不能使用电荷耦合器件(CCD)相机测量多个荧光团，这比传统的聚合酶链式反应或其他实时聚合酶链式反应仪器具有更高的精密度和准确度。这种准确性和精密度的提高对于单核苷酸多态(SNP)的可靠高通量数据分析、微阵列RNA表达分析的定量验证以及全基因组染色质免疫沉淀分析的定量分析至关重要。该系统的独特功能将使八个主要用户受益，并为超过35名用户提供Wistar基因组学核心增强功能，占Wistar研究所的约50%，并大量使用宾夕法尼亚大学的基础和临床研究人员。该仪器的所有主要用户都得到了美国国立卫生研究院的赠款支持和需要增加阵列样本实时聚合酶链式反应分析能力的项目。一个由Wistar研究所主要用户组成的咨询委员会将监督该仪器的操作、维护和公平使用。该仪器将被整合到Wistar基因组学核心设施中，该设施成立于1996年，致力于基因组研究的系统生物学计划和技术开发的长期发展。

DESCRIPTION (provided by applicant): Funds are requested for the purchase of an automated high-throughput real-time PCR system manufactured by Applied Biosystems. The 7900 HT Fast Real-Time 384/96 Well with custom Zymark Twister Robot for automatic plate loading capability will be located in the Wistar Institute Genomics Facility. There is currently no capability of high throughput automated real-time PCR available at the Wistar Institute. There is also no capability of measuring multiple fluorophores with a charge-coupled device (CCD) camera, which allows significantly higher precision and accuracy than traditional PCR or other real time PCR instruments. This increase in accuracy and precision is essential for reliable high throughput data analysis of single nucleotide polymorphisms (SNP), for quantitative verification of microarray RNA expression analysis, and for quantitative analysis of genome-wide chromatin immunoprecipitation analysis. The unique features of this system will benefit eight major users and provide the Wistar Genomics Core increased capabilities for over 35 users, representing ~50% of the Wistar Institute and substantial usage from basic and clinical investigators at the University of Pennsylvania. All major users of this instrument have NIH grant support and projects requiring increased capacity for real time PCR analysis of array samples. An advisory committee comprised of the major users from the Wistar Institute will oversee the operation, maintenance, and equitable access of this instrument. This instrument will be integrated in the Wistar Genomics Core Facility, which was established in 1996 and is committed to long-term development of systems biology program and technology development for genomic studies.