HIGH RESOLUTION DIGITAL CAMERA FOR TEM: OVARIAN CANCER, BREAST CANCER

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：新墨西哥大学医学院的电子显微镜共享设施成立于1966年，由医学院、新墨西哥州和NIH/NCRR提供的设备、建设和翻新资金不断升级。2001年SIG的一项奖励提供了急需的资金，用于购买配备100万像素数码相机的新日立7500透射式电子显微镜。我们寻求资金，通过购买680万像素的ATM XR-60C相机，大幅升级数字成像系统，以便在日立7500上进行高分辨率、高对比度成像。这里描述的八个主要用户和四个次要用户来自五个不同的学术部门，他们参与了三个不同的SOM研究计划：信号转导、黏附和贩运、分子遗传学和神经科学。七名主要调查人员领导着NIH资助的实验室。为这一应用程序提供资金将使其能够获得出版所需的高质量数字图像，并将具体支持对电子显微镜数据进行量化和统计分析的自动成像处理的需要。新相机将确保NIH资助的新墨西哥大学细胞和分子生物学以及神经科学方面的电子显微镜研究项目的持续生产力。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Electron Microscopy Shared Facility in the University of New Mexico School of Medicine was established in 1966 and has been continuously upgraded by equipment, construction and renovations funds from the School of Medicine, the State of New Mexico and NIH/NCRR. A SIG award in 2001 provided critically needed funds to purchase a new Hitachi 7500 transmission electron microscope equipped with a 1 megapixel digital camera. We seek funds to substantially upgrade the digital imaging system through the purchase of a 6.8 megapixel ATM XR-60C camera, for high resolution, high contrast imaging on the Hitachi 7500. The eight major and four minor users described here come from five different academic departments and participate in three different SOM research programs, Signal Transduction, Adhesion and Trafficking, Molecular Genetics, and Neurosciences. Seven of the major investigators direct NIH-funded laboratories. Funding for this application will permit acquisition of high quality digital images required for publication and will specifically support needs for automated imaging processing for quantitation and statistical analysis of electron microscopy data. The new camera will ensure the continued productivity of NIH-funded electron microscopy-based research programs in cellular and molecular biology and neurosciences at the University of New Mexico.