Protein Expression & Proteomics Resource

能够同时识别，定量和分析不同蛋白质之间的大量蛋白质混合物 细胞和组织的功能状态是蛋白质组学的最终目标，并且具有识别 在癌症生物学中重要的信号通路和复杂蛋白质网络。由于极端的 通过翻译后修饰（PTM）和RNA加工的蛋白质表达的多样性， 和表达的宽动态范围，不存在用于定量差异分析的单一平台。 全局蛋白表达。 因此，这一共享资源将整合三种设施和技术方法， 综合蛋白质组学的努力，由癌症中心：1）蛋白质化学/MS为基础的蛋白质组学， 单个蛋白质的鉴定和分析（PTM-翻译后分析）; 2） 杆状病毒系统中的重组蛋白和来自B细胞的单克隆抗体（MAbs） 杂交瘤，和3）分离、鉴定和基于MS的大蛋白复合物分析，或 “interactomes”。蛋白质化学/基于MS的蛋白质组学组件，在Richard博士的指导下 库克，自1989年以来一直作为贝勒的核心设施存在。重组蛋白表达组件 在2005年9月，随着Dean Edwards博士被招募到贝勒大学，开始了新的服务。的 interactome，或路径发现，组件将作为一个新的服务在博士的指导下启动。 秦军，公认的免疫分离和MS鉴定调节蛋白复合物的领导者。 除了指导杆状病毒/单克隆抗体机构外，Edwards博士还将担任 共享资源，以整合这三个组成部分之间的科学和行政互动。 蛋白质化学/MS为基础的蛋白质组学设施是一个成功的和具有成本效益的资源，提供 2005年，该研究为67名贝勒研究人员提供了服务，其中40%是癌症中心的成员。的 杆状病毒/单克隆抗体设施自2005年9月以来才开始运作， 为11名调查员提供服务，并安排了6个项目。全局差异蛋白表达谱 将通过结合二维凝胶分离蛋白质作为未来的服务来开发 电泳或液相色谱与高通量质谱联用。此外，我们计划开发 基于Luminex流式细胞荧光珠技术的多重免疫测定系统。

The ability to simultaneously identify, quantitate, and analyze a large mixture of proteins between different functional states of cells and tissues is the ultimate goal of proteomics, and has the potential to identify signaling pathways and complex protein networks important in cancer biology. Because of the extremely diverse nature of protein expression through post-translational modifications (PTM) and RNA processing, and the wide dynamic range of expression, no single platform exists for quantitative differential analysis of global protein expression. This Shared Resource will therefore integrate three facilities and technological approaches to create a comprehensive proteomic effort by the BCM Cancer Center: 1) protein chemistry/ MS-based proteomics for identification and analysis (PTM-postranslational analysis) of individual proteins; 2) expression of recombinant proteins in the baculovirus system and as monoconal antibodies (MAbs) from B-cell hybridomas, and 3) isolation, identification, and MS-based analysis of large protein complexes or "interactomes". The Protein Chemistry/MS-Based Proteomics component, under the direction of Dr. Richard Cook, has existed as a core facility at Baylor since 1989. The Recombinant Protein Expression components were started as new services in September of 2005 with the recruitment of Dr. Dean Edwards to Baylor. The interactome, or Pathway Discovery, component will be started as a new service under the direction of Dr. Jun Qin, a recognized leader in immuno-isolation and identification of regulatory protein complexes by MS. In addtion to directing the Baculovirus/MAb facility, Dr. Edwards will serve as the overall Leader of the Shared Resource to integrate the scientiific and administrative interactions among these three components. The Protein Chemistry/MS-Based Proteomic facility is a successful and cost effective resource that provided services for 67 Baylor investigators in 2005, of which 40% were Cancer Center members. The Baculovirus/MAb facility has only been in operation since September of 2005 and has already provided services for 11 investigators and has six projects scheduled. Global differential protein expression profiling will be developed as a future service by combining separation of proteins by two-dimensional gel electrophoresis or liquid chromatography coupled with high-through put MS. Additionally, we plan to develop multiplex immunoassay systems based on Luminex flow cytometric fluorescence bead technology.